The vitamin riboflavin and its derivative lumichrome activate the LasR bacterial quorum-sensing receptor.
The vitamin riboflavin and its derivative lumichrome activate the LasR bacterial quorum-sensing receptor.
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DOI：
10.1094/mpmi-21-9-1184
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发表时间：
2008-09
期刊：
影响因子：
--
通讯作者：
Phillips DA
中科院分区：
文献类型：
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作者：
Rajamani S;Bauer WD;Robinson JB;Farrow JM 3rd;Pesci EC;Teplitski M;Gao M;Sayre RT;Phillips DA
许多细菌利用群体感应（quorum sensing，QS）作为细胞间信号传导机制来调节局部种群的基因表达。反过来，植物和藻类宿主分泌模拟细菌QS信号的化合物，使这些宿主能够操纵细菌中QS调节的基因表达。光色素是维生素核黄素的衍生物，从衣原体的培养物中纯化并化学鉴定为能够刺激铜绿假单胞菌LasR QS受体的QS信号模拟化合物。LasR通常识别N-酰基高丝氨酸内酯（阿勒）信号，N-3-氧代-十二烷酰基高丝氨酸内酯（3-氧代-C12-HSL）。在生物测定中，真实的光色素和核黄素刺激LasR受体，并且在凝胶位移实验中，光色素激活LasR。阿勒结合所需的LasR残基中的氨基酸取代改变了对AHL和光色素/核黄素的反应。这些结果和对接研究表明，LasR的阿勒结合口袋识别AHL和结构上不同的发光色素/核黄素。细菌、植物和藻类通常分泌核黄素和/或光色素，这提高了这些化合物可以作为QS信号或作为能够用LasR样受体操纵细菌中的QS的界间信号模拟物的可能性。
Many bacteria use quorum sensing (QS) as an intercellular signaling mechanism to regulate gene expression in local populations. Plant and algal hosts, in turn, secrete compounds that mimic bacterial QS signals, allowing these hosts to manipulate QS-regulated gene expression in bacteria. Lumichrome, a derivative of the vitamin riboflavin, was purified and chemically identified from culture filtrates of the alga Chlamydomonas as a QS signal-mimic compound capable of stimulating the Pseudomonas aeruginosa LasR QS receptor. LasR normally recognizes the N-acyl homoserine lactone (AHL) signal, N-3-oxo-dodecanoyl homoserine lactone (3-oxo-C12-HSL). Authentic lumichrome and riboflavin stimulated the LasR receptor in bioassays, and lumichrome activated LasR in gel shift experiments. Amino acid substitutions in LasR residues required for AHL binding altered responses to both AHLs and lumichrome/riboflavin. These results and docking studies indicate that the AHL binding pocket of LasR recognizes both AHLs and the structurally dissimilar lumichrome/riboflavin. Bacteria, plants and algae commonly secrete riboflavin and/or lumichrome, raising the possibility that these compounds could serve as either QS signals or as interkingdom signal-mimics capable of manipulating QS in bacteria with a LasR-like receptor.