GLUTAMIC-ACID DECARBOXYLASE IN TUBULES AND GLOMERULI ISOLATED FROM RAT-KIDNEY CORTEX
GLUTAMIC-ACID DECARBOXYLASE IN TUBULES AND GLOMERULI ISOLATED FROM RAT-KIDNEY CORTEX
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DOI：
10.1111/j.1432-1033.1985.tb09109.x
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发表时间：
1985-01-01
期刊：
影响因子：
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通讯作者：
HELWIG, JJ
中科院分区：
文献类型：
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作者：
BURGMEIER, N;ZAWISLAK, R;HELWIG, JJ
4-氨基丁酸（GABA）的合成进行了检查，在纯化的肾小球和肾小管的大鼠肾皮质孵育的存在下，[2，3 - 3 H2]谷氨酸。使用阴离子交换树脂将形成的GABA与谷氨酸分离，并通过自动氨基酸分析仪进行鉴定。在肾皮质中，只有小管能够形成GABA（35.0 nmol mg-1h-1）;在肾小球制备物中发现的剩余GABA合成最可能归因于皮质小管的污染（9%），如通过测定已知的小管标记酶（L-γ-GABA）所示。谷氨酰转移酶）。羟胺（1 mM）和乙醇胺-O-硫酸盐（10 mM），脑GABA形成和GABA催化剂的众所周知的抑制剂，抑制肾小管GABA形成分别为100%和44%。
4-Aminobutyric acid (GABA) synthesis was examined in purified glomeruli and tubules of rat kidney cortex that were incubated in the presence of [2,3-3H2]glutamate. The GABA that was formed was separated from glutamate using anion-exchange resin, and identified by means of an automatic amino acid analyser. In the renal cortex only the tubules were able to form GABA (35.0 nmol mg-1h-1); the remaining GABA synthesis found in the glomerular preparations can most probably be attributed to a contamination by cortical tubules (9%), as shown by determination of a known tubular marker enzyme (L-.gamma.-glutamyltransferase). Hydroxylamine (1 mM) and ethanolamine-O-sulfate (10 mM), well-known inhibitors of cerebral GABA formation and GABA catabolism respectively, inhibited renal tubular GABA formation at 100% and 44% respectively.