INORGANIC AND ORGANIC PHOSPHATE MEASUREMENTS IN THE NANOMOLAR RANGE
INORGANIC AND ORGANIC PHOSPHATE MEASUREMENTS IN THE NANOMOLAR RANGE
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DOI：
10.1016/0003-2697(87)90649-x
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发表时间：
1987-02-15
影响因子：
2.9
通讯作者：
MANNAERTS, GP
中科院分区：
文献类型：
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作者：
VANVELDHOVEN, PP;MANNAERTS, GP
基于孔雀石绿与磷钼酸盐在酸性条件下形成络合物，建立了测定纳摩尔范围内无机正磷酸盐的方法。需要添加聚乙烯醇来稳定染料-磷钼酸络合物。该方法具有操作简便、试剂稳定、灵敏度高等优点。由于测定中允许的酸度很高(0.9n H_2SO_4)，该方法可以很容易地测定湿法消化后从磷蛋白和磷脂等有机化合物中释放出来的纳摩尔量的磷。
A procedure, based on the complex formation of malachite green with phosphomolybdate under acidic conditions, to measure inorganic orthophosphate in the nanomolar range is described. The addition of polyvinyl alcohol is required to stabilize the dye-phosphomolybdate complex. The advantages of the assay are simplicity, stability of the reagents, and high sensitivity. Due to the high permissible acidity in the assay (0.9 n H2SO4), the method can be adapted easily to measure nanomolar amounts of phosphate, liberated from organic compounds like phosphoproteins and phospholipids after wet digestion.