Mechanical Stress Acts via Katanin to Amplify Differences in Growth Rate between Adjacent Cells in Arabidopsis
Mechanical Stress Acts via Katanin to Amplify Differences in Growth Rate between Adjacent Cells in Arabidopsis
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DOI：
10.1016/j.cell.2012.02.048
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发表时间：
2012-04-13
期刊：
影响因子：
64.5
通讯作者：
Hamant, Olivier
中科院分区：
文献类型：
--
作者：
Uyttewaal, Magalie;Burian, Agata;Hamant, Olivier
在组织中存在的弥漫性形态梯度支持生长是局部均匀的观点。在这里，我们挑战这种观点：我们使用高分辨率定量方法来揭示茎顶端分生组织（植物干细胞生态位）中相邻细胞之间的显著生长变异性。这种变异性大大降低了突变受损的微管切断蛋白katanin。突变体中的主要形状缺陷可能与生长异质性的局部降低有关。我们表明，katanin是所需的细胞的能力，以响应生长所产生的机械力。这为微管动力学允许细胞有效地响应机械力的模型提供了基础。这反过来又可以放大局部生长率梯度，产生更多的异质生长和支持形态发生。
The presence of diffuse morphogen gradients in tissues supports a view in which growth is locally homogenous. Here we challenge this view: we used a high-resolution quantitative approach to reveal significant growth variability among neighboring cells in the shoot apical meristem, the plant stem cell niche. This variability was strongly decreased in a mutant impaired in the microtubule-severing protein katanin. Major shape defects in the mutant could be related to a local decrease in growth heterogeneity. We show that katanin is required for the cell's competence to respond to the mechanical forces generated by growth. This provides the basis for a model in which microtubule dynamics allow the cell to respond efficiently to mechanical forces. This in turn can amplify local growth-rate gradients, yielding more heterogeneous growth and supporting morphogenesis.