Screening microalgae for some potentially useful agricultural and pharmaceutical secondary metabolites
Screening microalgae for some potentially useful agricultural and pharmaceutical secondary metabolites
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DOI：
10.1023/b:japh.0000047789.34883.aa
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发表时间：
2004-08-01
影响因子：
3.3
通讯作者：
Németh, L
中科院分区：
文献类型：
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作者：
Ördög, V;Stirk, WA;Németh, L
采用纸片扩散法对近200株微藻（174株绿藻和23株蓝细菌）进行了抗细菌、丝状真菌和酵母菌的筛选。从该初步筛选中，选择了来自三个属（链藻属、小球藻属和栅列藻属）的10个绿藻菌株，因为它们具有高的抗微生物活性。对这10株菌株进行部分纯化，并使用MIC抗菌和微量滴定IC 50抗癌测定进行测试。这些预选的藻类菌株显示出对革兰氏阳性（10个物种中的9个）和革兰氏阴性（10个物种中的7个）细菌的高发生率的抗菌活性。该提取物对某些肿瘤细胞系也有效。
Nearly two hundred microalgal strains ( 174 Chlorophyta and 23 Cyanobacteria) were screened against some bacteria, filamentous fungi and yeasts using a disc-diffusion type bioassay. From this initial screening, 10 Chlorophyta strains from three genera ( Desmococcus, Chlorella and Scenedesmus) were selected because of their high antimicrobial activity. These 10 strains were partially purified and tested using MIC antimicrobial and microtiter IC50 anticancer assays. These preselected algal strains showed a high incidence of antibacterial activity against both Gram-positive ( 9 out of 10 species) and Gram-negative ( 7 out of 10 species) bacteria. The extracts were also effective against some tumour cell lines.