Detection of biological responses to low-dose radiation in humans
Detection of biological responses to low-dose radiation in humans
复制标题
检测人体对低剂量辐射的生物反应
DOI：
10.1016/j.freeradbiomed.2022.04.006
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发表时间：
2022
影响因子：
7.4
通讯作者：
Tokonami Shinji
中科院分区：
文献类型：
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作者：
Yamaguchi Masaru;Tatara Yota;Nugraha Eka Djatnika;Ramadhani Dwi;Tamakuma Yuki;Sato Yoshiaki;Miura Tomisato;Hosoda Masahiro;Yoshinaga Shinji;Syaifudin Mukh;Kashiwakura Ikuo;Tokonami Shinji
人们认为，很难检测人体受到低于约100毫希沃特的低剂量辐射的生物效应。对从印度尼西亚马木居一个新发现的高水平天然本底辐射区（年有效剂量约为50 mSv y−1）和正常水平区（1.22 mSv y−1）的居民采集的血样进行了血清蛋白质组学分析和氧化修饰谱分析，许多人在那里生活了几代人。在人血清白蛋白的氨基酸序列中发现了剂量依赖性的氧化修饰，特别是第162位和第356位酪氨酸残基以及第111位和第470位甲硫氨酸残基。它不仅可用作评估是否存在辐射照射的生物标志物，而且可用于生物体中慢性辐射的剂量预测。这些结果表明，微量的辐射照射记录在血清白蛋白，有一种新的方法，可以评估低于100毫希的生物反应的可能性。
It has been considered difficult to detect the biological effects of low-dose radiation exposure below approximately 100 mSv in humans. Serum proteomic analysis and oxidative modification profiling were conducted with blood samples collected from residents of a newly discovered high-level natural background radiation area (annual effective dose approximately 50 mSv y−1) and normal-level area (1.22 mSv y−1) in Mamuju, Indonesia, where many people have been living for generations. Dose-dependent oxidative modifications in amino acid sequences of human serum albumin, especially the 162ndand 356thtyrosine residues and 111thand 470thmethionine residues, were found. None of these findings have been reported in humans exposed to chronic low-dose radiation. It can be used as a biomarker not only for the assessment of the presence or absence of radiation exposure but also for dose prediction in living organisms for chronic radiation. These results suggest that traces of radiation exposure are recorded in serum albumin and that there is a possibility of a new methodology that can evaluate biological responses below 100 mSv.