Collection of X-Ray Diffraction Data from Macromolecular Crystals.
Collection of X-Ray Diffraction Data from Macromolecular Crystals.
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DOI：
10.1007/978-1-4939-7000-1_7
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Dauter Z
中科院分区：
文献类型：
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作者：
Dauter Z
衍射数据采集是晶体结构分析的最后实验阶段。所有后续步骤主要涉及计算机计算。最佳的测量和准确的数据使结构的求解和改进变得更容易，并导致对最终模型的更真实的解释。本文讨论了从大分子晶体中采集数据的重要因素，并提出了适用于各种应用的策略，如分子置换、反常位相、原子分辨率精化等。文中还讨论了判断衍射数据质量的标准。
Diffraction data acquisition is the final experimental stage of the crystal structure analysis. All subsequent steps involve mainly computer calculations. Optimally measured and accurate data make the structure solution and refinement easier and lead to more faithful interpretation of the final models. Here, the important factors in data collection from macromolecular crystals are discussed and strategies appropriate for various applications, such as molecular replacement, anomalous phasing, atomic-resolution refinement etc., are presented. Criteria useful for judging the diffraction data quality are also discussed.