Exhalation of Respiratory Viruses by Breathing, Coughing, and Talking
Exhalation of Respiratory Viruses by Breathing, Coughing, and Talking
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DOI：
10.1002/jmv.21556
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发表时间：
2009-09-01
影响因子：
12.7
通讯作者：
Tovey, Euan R.
中科院分区：
文献类型：
--
作者：
Stelzer-Braid, Sacha;Oliver, Brian G.;Tovey, Euan R.
缺乏有关感染者产生病毒气溶胶的定量信息。使用新型口罩对 50 名受试者因咳嗽、说话和呼吸产生的呼出气溶胶进行了采样，并使用 PCR 分析了 9 种呼吸道病毒。还通过细胞培养检查了 10 名鼻病毒 PCR 呈阳性受试者的呼出样本中是否存在该病毒。在50名受试者中，33名有上呼吸道感染症状的受试者中，21名通过PCR检测到至少一种病毒，而在17名无症状受试者中，4名通过PCR检测到病毒。总体而言，19 名受试者检测到鼻病毒，4 名受试者检测到流感，2 名受试者检测到副流感病毒，1 名受试者检测到人类偏肺病毒。两名受试者同时感染。在 25 名鼻粘液病毒呈阳性的受试者中，在 12 份呼吸样本、8 份说话样本和 2 份咳嗽样本中检测到了相同的病毒类型。在 10 名受试者的呼气样本中，有 2 名受试者检测到感染性鼻病毒。这些数据进一步证明，呼吸可能是携带感染性病毒的可吸入颗粒的来源。 J.医学。病毒。 81:1674-1679, 2009。(C) 2009 Wiley-Liss, Inc.
There is a lack of quantitative information about the generation of virus aerosols by infected subjects. The exhaled aerosols generated by coughing, talking, and breathing were sampled in 50 subjects using a novel mask, and analyzed using PCR for nine respiratory viruses. The exhaled samples from a subset of 10 subjects who were PCR positive for rhinovirus were also examined by cell culture for this virus. Of the 50 subjects, among the 33 with symptoms of upper respiratory tract infections, 21 had at least one virus detected by PCR, while amongst the 17 asymptomatic subjects, 4 had a virus detected by PCR. Overall, rhinovirus was detected in 19 subjects, influenza in 4 subjects, parainfluenza in 2 subjects, and human metapneumovirus in 1 subject. Two subjects were co-infected. Of the 25 subjects who had virus-positive nasal mucus, the same virus type was detected in 12 breathing samples, 8 talking samples, and in 2 coughing samples. In the subset of exhaled samples from 10 subjects examined by culture, infective rhinovirus was detected in 2. These data provide further evidence that breathing may be a source of respirable particles carrying infectious virus. J. Med. Virol. 81:1674-1679, 2009. (C) 2009 Wiley-Liss, Inc.