Cardiac sarcoplasmic reticulum function in insulin- or carnitine-treated diabetic rats.
Cardiac sarcoplasmic reticulum function in insulin- or carnitine-treated diabetic rats.
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胰岛素或肉碱治疗的糖尿病大鼠的心脏肌浆网功能。
DOI：
10.1152/ajpheart.1983.245.6.h969
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
J. McNeill
中科院分区：
文献类型：
--
作者：
G. Lopaschuk;A. G. Tahiliani;R. Vadlamudi;S. Katz;J. McNeill
用胰岛素和D，左旋肉碱分别治疗链脲霉素诱导的糖尿病大鼠，测定其心肌肌浆网（SR）功能和长链酰基肉碱（LC） SR水平。与对照组大鼠相比，未经治疗的糖尿病大鼠心脏SR中atp依赖性钙转运明显下降。糖尿病大鼠心脏中LC酰基肉碱的SR水平也显著升高。在离体工作心脏装置上测定的各种心功能参数（左心室发育压、+dP/dT和-dP/dT）在未治疗的糖尿病大鼠中被发现下降。在整个研究期间，用胰岛素或D -左旋肉碱治疗的糖尿病大鼠心脏SR分离后，SR膜相关的LC酰基肉碱水平没有升高，SR钙转运活性也没有下降。胰岛素治疗的糖尿病大鼠的心脏功能与对照大鼠相似，但D，左旋肉碱治疗的糖尿病大鼠心脏功能仍然下降。提示LC酰基肉碱参与了糖尿病大鼠心脏SR功能的损害。然而，在这些动物身上发现的心脏功能下降一定是由其他因素造成的。
Cardiac sarcoplasmic reticulum (SR) function and SR levels of long-chain (LC) acylcarnitines were determined in streptozotocin-induced diabetic rats treated with insulin or D,L-carnitine. ATP-dependent calcium transport was significantly depressed in cardiac SR isolated from untreated diabetic rats compared with control rats. Diabetic rat cardiac SR levels of LC acylcarnitines were also significantly elevated. Various parameters of heart function (left ventricular developed pressure, +dP/dT, and -dP/dT), as determined on an isolated working heart apparatus, were found to be depressed in untreated diabetic rats. Cardiac SR isolated from diabetic rats treated throughout the study period with insulin or D,L-carnitine did not have elevated levels of LC acylcarnitines associated with SR membrane nor was SR calcium transport activity depressed. Heart function in the diabetic rats treated with insulin was similar to control rat hearts but heart function remained depressed in diabetic rats treated with D,L-carnitine. The data suggest that the LC acylcarnitines are involved in the observed impairment of cardiac SR function in diabetic rats. Other factors, however, must be contributing to the depression in heart function noted in these animals.