Higher-Order Interactions Dampen Pairwise Competition in the Zebrafish Gut Microbiome.
Higher-Order Interactions Dampen Pairwise Competition in the Zebrafish Gut Microbiome.
复制标题
高阶相互作用抑制斑马鱼肠道微生物组中的成对竞争。
DOI：
10.1128/mbio.01667-20
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发表时间：
2020-10-13
期刊：
影响因子：
6.4
通讯作者：
Parthasarathy R
中科院分区：
文献类型：
--
作者：
Sundarraman D;Hay EA;Martins DM;Shields DS;Pettinari NL;Parthasarathy R
了解脊椎动物肠道环境中不同微生物群落组成的规律，将增强我们操纵这些群落用于治疗目的的能力。合成的微生物群落，组装从特定的组合微生物物种在无菌动物，允许调查的基本问题，是否可以预测多物种群落组成的基础上，对物种之间的相互作用的综合影响。如果是这样的话，这种可预测性将使自下而上构建具有所需物种的社区成为可能。如果不是，那么明显的高阶相互作用意味着新兴的社区水平特征是至关重要的。我们的研究结果使用多达五种共存的原生细菌物种在斑马鱼幼虫，一种模式脊椎动物，提供了高阶相互作用的实验证据，而且，这些相互作用促进肠道中微生物物种的共存。动物肠道中的微生物群落由多种物种组成，它们在宿主发育、健康和疾病中起着重要作用。由于这些社区的复杂性和在原地表征它们的困难，微生物组成的决定因素在很大程度上仍然未知。此外，目前还不清楚许多多物种的财团是否可以预测其物种水平的化妆的基础上的理解成对物种的相互作用，或是否需要高阶相互作用来解释紧急组成。为了解决这一问题，我们研究了斑马鱼幼鱼的肠道微生物，最初提出无菌，允许引入细菌物种的控制组合。使用解剖和平板试验，我们证明了一至五个细菌物种的社区的建设，并表明，从两个物种的竞争结果无法预测在更复杂的社区物种丰度。随着多个物种的存在，细菌间的相互作用变得越来越弱，这表明脊椎动物肠道中的高阶相互作用稳定了复杂的群落。
Understanding the rules governing the composition of the diverse microbial communities that reside in the vertebrate gut environment will enhance our ability to manipulate such communities for therapeutic ends. Synthetic microbial communities, assembled from specific combinations of microbial species in germfree animals, allow investigation of the fundamental question of whether multispecies community composition can be predicted solely based on the combined effects of interactions between pairs of species. If so, such predictability would enable the construction of communities with desired species from the bottom up. If not, the apparent higher-order interactions imply that emergent community-level characteristics are crucial. Our findings using up to five coexisting native bacterial species in larval zebrafish, a model vertebrate, provide experimental evidence for higher-order interactions and, moreover, show that these interactions promote the coexistence of microbial species in the gut. The microbial communities resident in animal intestines are composed of multiple species that together play important roles in host development, health, and disease. Due to the complexity of these communities and the difficulty of characterizing them in situ, the determinants of microbial composition remain largely unknown. Further, it is unclear for many multispecies consortia whether their species-level makeup can be predicted based on an understanding of pairwise species interactions or whether higher-order interactions are needed to explain emergent compositions. To address this, we examine commensal intestinal microbes in larval zebrafish, initially raised germfree, to allow the introduction of controlled combinations of bacterial species. Using a dissection and plating assay, we demonstrate the construction of communities of one to five bacterial species and show that the outcomes from the two-species competitions fail to predict species abundances in more complex communities. With multiple species present, interbacterial interactions become weaker, suggesting that higher-order interactions in the vertebrate gut stabilize complex communities.