Transfer of antigen-pulsed dendritic cells induces specific T-Cell proliferation and a therapeutic effect against long-term Helicobacter pylori infection in mice
Transfer of antigen-pulsed dendritic cells induces specific T-Cell proliferation and a therapeutic effect against long-term Helicobacter pylori infection in mice
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DOI：
10.1128/iai.74.2.984-993.2006
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发表时间：
2006-02-01
影响因子：
3.1
通讯作者：
Nishizono, A
中科院分区：
文献类型：
--
作者：
Otsu, S;Gotoh, K;Nishizono, A
幽门螺杆菌引起胃的持续感染，并导致慢性胃炎和消化性溃疡。Jaws 11细胞，来自小鼠骨髓，用活的或福尔马林杀死的或全细胞超声（WCS）的H。幽门。代表性的细胞表面分子在Jaws 11细胞上以实质水平表达，表明用三种H. pylori抗原无显著差异。H. pylori WCS脉冲的Jaws II细胞分泌大量的肿瘤坏死因子α到培养上清液中。暴露于WCS脉冲的Jaws 11细胞的初始T细胞在体外显示出显著的增殖和γ干扰素（IFN-γ）和白细胞介素-10（IL-10）产生。在用WCS脉冲的Jaws 11细胞处理的小鼠中观察到定殖细菌数量减少2个对数;然而，在用其他H. pylori抗原。在用WCS脉冲的Jaws 11细胞处理的小鼠的胃中观察到IFN-γ和IL-10的上调产生，这与体外获得的结果一致。胃炎评分和H。用三种不同的H. pylori抗原。结果表明，Th 1细胞介导的免疫结合Th 2细胞介导的免疫在减少慢性H.幽门感染
Helicobacter pylori causes persistent infection of the stomach and results in chronic gastritis and peptic ulcers. Jaws 11 cells, derived from mouse bone marrow, were pulsed with live or formalin-killed or whole-cell sonicates (WCS) of H. pylori. Representative cell surface molecules were expressed at substantial levels on Jaws 11 cells, indicating that appropriate maturation of the cells was achieved with the three H. pylori antigens without any significant differences. H. pylori WCS-pulsed Jaws II cells secreted a significant amount of tumor necrosis factor alpha into the culture supernatant. The naive T cells exposed to the WCS-pulsed Jaws 11 cells showed significant proliferation and gamma interferon (IFN-gamma) and interleukin-10 (IL-10) production in vitro. A 2-log reduction in the number of colonizing bacteria was observed in the mice treated with the WCS-pulsed Jaws 11 cells; however, no significant reductions were achieved in mice treated with Jaws 11 cells pulsed with other H. pylori antigens. Up-regulated production of IFN-gamma and IL-10 was observed in the stomachs of the mice treated with the WCS-pulsed Jaws 11 cells, which is consistent with the result obtained in vitro. There were no differences in gastritis scores or H. pylori-specific antibody titers among the mice treated with Jaws 11 cells pulsed with the three different H. pylori antigens. The results suggest that Th1 cell-mediated immunity in combination with Th2 cell-mediated immunity plays a role in reducing colonizing bacterial numbers in mice with chronic H. pylori infections.