DEP1 gene in wheat species with normal, compactoid and compact spikes.
DEP1 gene in wheat species with normal, compactoid and compact spikes.
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DOI：
10.1186/s12863-017-0583-6
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发表时间：
2017-12-28
期刊：
影响因子：
2.9
通讯作者：
Goncharov NP
中科院分区：
文献类型：
--
作者：
Vavilova V;Konopatskaia I;Kuznetsova AE;Blinov A;Goncharov NP
在水稻中，DEP1基因的一个变异导致直立的穗结构，维管束发育良好，每穗粒数增加，从而提高产量。有趣的是，DEP1的同系物也存在于其他谷物中，包括小麦和大麦（Hordeum vulgare），尽管它们不产穗，但产穗。在大麦中，HvDEP1等位基因在不同穗型和地理来源的品系之间没有差异，而在至少三个OsDEP1变体中已经被描述。本研究对单粒小麦、硬粒小麦、紧实小麦和细粒小麦这4种小麦品种的8个DEP1基因进行了研究，这些小麦品种的穗型分别为致密型、致密型和正常穗型。测定了8份材料DEP1第5外显子的核苷酸序列。获得的序列具有物种特异性。尽管存在种间多样性，但所有小麦序列编码的多肽大小相同，类似于T. aestivum、T. urartu和H. vulgare中DEP1同源物的第5外显子。为了进一步研究，我们研究了这四个物种的DEP1基因的全长序列。从aestivum、T. urartu和Aegilops tauschii基因组序列中分离到DEP1全长基因组拷贝。四倍体硬粒小麦的基因组包含两个DEP1变体，分别来自A和B基因组。在六倍体小麦（T. aestivum， T. compactum， T. spelta）中，该基因分别来自A、B和D基因组。二倍体小麦单粒小麦和乌拉尔小麦的DEP1基因不同。单球绦虫中DEP1基因的一个前体似乎起源于野生的boeoticum。在被试小麦品种中，紧密穗型（或紧密样穗型）和正常穗型之间的核苷酸序列未检测到dep1相关的差异。因此，DEP1基因并不直接参与小麦穗结构的调控。本文的在线版本（10.1186/s12863-017-0583-6）包含补充材料，授权用户可使用。
In rice, a variant of DEP1 gene results in erect panicle architecture, well-developed vascular bundles, an increase in the number of grains per panicle and a consequent increase in the grain yield. Interestingly, DEP1 homologs are present in the other cereals including species of wheat and barley (Hordeum vulgare), even though they do not produce panicles but spikes. In barley, HvDEP1 alleles do not differ between strains of various ear types and geographic origins, while in at least three OsDEP1 variants have been described. In this work, we have studied the DEP1 gene from eight accessions which belong to four wheat species, T. monococcum, T. durum, T. compactum, and T. spelta, with either compact, compactoid or normal spike phenotypes. The nucleotide sequences of the 5th exon of DEP1 were determined for all eight accessions. Obtained sequences were species specific. Despite the interspecies diversity, all wheat sequences encoded polypeptides of the same size, similarly to the 5th exons of the DEP1 homologs in T. aestivum, T. urartu, and H. vulgare. For further study, the full-length sequences of the DEP1 gene for all four species were studied. The full-length DEP1 genomic copies were isolated from the genomic sequences of T. aestivum, T. urartu, and Aegilops tauschii. The genome of tetraploid wheat T. durum contains two variants of the DEP1 originating from A and B genomes. In the hexaploid wheats T. aestivum, T. compactum, and T. spelta, three variants of this gene originating from A, B, and D genomes were detected. DEP1 genes of the diploid wheats T. monococcum and T. urartu differ. It seems that a precursor of the DEP1 gene in T. monococcum originates from the wild progenitor T. boeoticum. No DEP1-related differences of nucleotide sequences between the compact (or compactoid) and normal spike phenotypes in the tested wheat species were detected. Therefore, DEP1 gene does not directly participate in the control of the spike architecture in wheats. The online version of this article (10.1186/s12863-017-0583-6) contains supplementary material, which is available to authorized users.
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影响因子：
0.6
作者：
Goncharov, NP;Gaidalenok, RF
通讯作者：
Gaidalenok, RF
DOI：
10.1073/pnas.1110552108
发表时间：
2011-11-15
影响因子：
11.1
作者：
Zhang, Zengcui;Belcram, Harry;Chalhoub, Boulos
通讯作者：
Chalhoub, Boulos
影响因子：
1.9
作者：
Belanger, Sebastien;Gauthier, Melanie;Belzile, Francois
通讯作者：
Belzile, Francois
影响因子：
6.5
作者：
Guindon, Stephane;Dufayard, Jean-Francois;Gascuel, Olivier
通讯作者：
Gascuel, Olivier
影响因子：
0.6
作者：
Goncharov, NP;Mitina, RL;Anfilova, NA
通讯作者：
Anfilova, NA