The development of an Arabidopsis model system for genome-wide analysis of polyploidy effects
The development of an Arabidopsis model system for genome-wide analysis of polyploidy effects
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DOI：
10.1111/j.1095-8312.2004.00351.x
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发表时间：
2004-08-01
影响因子：
1.9
通讯作者：
Osborn, TC
中科院分区：
文献类型：
--
作者：
Chen, ZJ;Wang, JL;Osborn, TC
拟南芥是一个模式系统，不仅用于研究植物生物学的许多方面，而且还用于理解与基因组复制和多倍化相关的快速进化过程的机制。虽然在动物种间杂交往往是不育的，非整倍体与疾病综合征，拟南芥同源多倍体和异源多倍体发生在自然界中，可以很容易地在实验室中形成，提供了一个有吸引力的系统比较相对“年轻”和“建立”或“古老”的多倍体之间的基因表达和基因组结构的变化。强大的反向和正向遗传学在拟南芥提供了一个特殊的手段，基因和基因组复制的调控机制可能会被揭示。此外，拟南芥基因组完全测序;编码和非编码序列都是可用的。我们已经开发了斑点寡基因和染色体微阵列使用完整的拟南芥基因组序列。寡基因微阵列由约26 000个70-mer寡核苷酸组成，所述寡核苷酸是从拟南芥中所有注释的基因设计的，并且染色体微阵列包含从染色体区域或4号染色体的完整序列扩增的1 kb基因组平铺片段。我们已经证明了微阵列的效用，在拟南芥多倍体和相关物种的基因表达，基因组组织和染色质结构的变化进行全基因组分析。(C)2004年，伦敦林奈学会。
Arabidopsis is a model system not only for studying numerous aspects of plant biology, but also for understanding mechanisms of the rapid evolutionary process associated with genome duplication and polyploidization. Although in animals interspecific hybrids are often sterile and aneuploids are related to disease syndromes, both Arabidopsis autopolyploids and allopolyploids occur in nature and can be readily formed in the laboratory, providing an attractive system for comparing changes in gene expression and genome structure among relatively 'young' and 'established' or 'ancient' polyploids. Powerful reverse and forward genetics in Arabidopsis offer an exceptional means by which regulatory mechanisms of gene and genome duplication may be revealed. Moreover, the Arabidopsis genome is completely sequenced; both coding and non-coding sequences are available. We have developed spotted oligo-gene and chromosome microarrays using the complete Arabidopsis genome sequence. The oligo-gene microarray consists of -26 000 70-mer oligonucleotides that are designed from all annotated genes in Arabidopsis, and the chromosome microarray contains 1 kb genomic tiling fragments amplified from a chromosomal region or the complete sequence of chromosome 4. We have demonstrated the utility of microarrays for genome-wide analysis of changes in gene expression, genome organization and chromatin structure in Arabidopsis polyploids and related species. (C) 2004 The Linnean Society of London.