PRODUCTION OF STABLE CYTOLYTIC T-CELL CLONES DIRECTED AGAINST AUTOLOGOUS HUMAN-MELANOMA
PRODUCTION OF STABLE CYTOLYTIC T-CELL CLONES DIRECTED AGAINST AUTOLOGOUS HUMAN-MELANOMA
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DOI：
10.1002/ijc.2910390320
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发表时间：
1987-03-15
影响因子：
6.4
通讯作者：
BOON, T
中科院分区：
文献类型：
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作者：
HERIN, M;LEMOINE, C;BOON, T
我们尝试优化针对自体黑色素瘤细胞系的稳定人溶细胞性 T 淋巴细胞克隆的生产。每周用含有人血清和 IL-2 的培养基中经照射的黑色素瘤细胞重新刺激 MLTC。 21 至 35 天后，与自体 EBV-B 细胞或 NK 靶标 K562 相比，在 6 名患者中有 5 名患者中，这些培养物表现出针对自体黑色素瘤细胞的优先细胞溶解活性。在第 7 天至第 28 天的不同时间，在含有 IL-2 和同种异体 EBV-B 细胞作为饲养细胞的培养基中进行 MLTC 应答细胞的有限稀释。这些应答细胞中大约 1% 产生裂解自体黑色素瘤细胞的 CTL 克隆，但不裂解 K562 或自体 B 细胞。大量 CTL 克隆可以在培养物中维持几个月，这些克隆保留了这种高活性的特异性，并且每周繁殖超过 5 倍。其中一些 CTL 克隆的生长依赖于自体黑色素瘤细胞的存在。对于一种黑色素瘤，使用自体 CTL 克隆可以识别肿瘤细胞上的 3 种不同抗原。
We have attempted to optimize the production of stable human cytolytic T lymphocyte clones directed against autologous melanoma cell lines. MLTC were restimulated every week with irradiated melanoma cells in medium containing human serum and IL-2. After 21 to 35 days, in 5 out of 6 patients, these cultures expressed a preferential cytolytic activity against the autologous melanoma cells, as compared to autologous EBV-B cells or NK target K562. Limiting dilution of MLTC responder cells was performed at times varying from days 7 to 28, in medium containing IL-2 and allogeneic EBV-B cells as feeders. Approximately 1% of these responder cells gave rise to CTL clones that lysed the autologous melanoma cells, but did not lyse K562 or autologous B cells. It was possible to maintain in culture for several months a large number of CTL clones that retained this specificity with high activity, and multiplied more than 5-fold every week. Some of these CTL clones were dependent on the presence of the autologous melanoma cells for their growth. With one melanoma, the use of autologous CTL clones made it possible to identify 3 different antigens on the tumor cells.