Cytochrome P-450 metabolic activity in embryonic and extraembryonic tissue lineages of mouse embryos.
Cytochrome P-450 metabolic activity in embryonic and extraembryonic tissue lineages of mouse embryos.
复制标题
小鼠胚胎胚胎和胚胎外组织谱系中的细胞色素 P-450 代谢活性。
DOI：
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发表时间：
1985
影响因子：
11.1
通讯作者：
S. Galloway
中科院分区：
文献类型：
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作者：
R. Pedersen;J. Meneses;A. Spindle;K. Wu;S. Galloway
小鼠桑椹胚，囊胚，胚胎和胚外组织层苯并[a]-芘代谢细胞色素P-450，使用姐妹染色单体交换试验。苯并[a]芘体外暴露增加了所有检测的遗传反应小鼠[BALB/cDub、C3 H/AnfCum和远交Dub：（ICR）品系]的囊胚中的姐妹染色单体交换，但未增加无反应AKR/J品系的囊胚中的姐妹染色单体交换。苯并[a]芘处理7 1/2天和8 1/2天（植入后阶段）的胚胎，无论是完整的还是作为单独的组织层，都增加了胚胎和胚外谱系组织中的姐妹染色单体交换-即，在胚胎中、在分离的胚胎外胚层中、在卵黄囊、绒毛膜、胚外外胚层和胚外内胚层层中。这些结果表明，细胞色素P-450在早期哺乳动物胚胎的大部分或所有组织中是活跃的。它可以代谢异生物质分子，在形态发生和器官发生开始时到达孕体，或者它在正常发育中可能有另一个尚未确定的作用。
Mouse morulae, blastocysts, and embryonic and extraembryonic tissue layers were examined for benzo[a]-pyrene metabolism by cytochrome P-450, using the sister chromatid exchange assay. Benzo[a]pyrene exposure in vitro increased sister chromatid exchanges in blastocysts of all genetically responsive mice examined [BALB/cDub, C3H/AnfCum, and outbred Dub:(ICR) strains] but not blastocysts of the nonresponsive AKR/J strain. Benzo[a]pyrene treatment of responsive 7 1/2- and 8 1/2-day (postimplantation-stage) embryos, either intact or as separate tissue layers, increased sister chromatid exchanges in tissues of both embryonic and extraembryonic lineages--i.e., in the embryo proper, in isolated embryonic ectoderm, and in yolk sac, chorion, extraembryonic ectoderm, and extraembryonic endoderm layers. These results indicate that cytochrome P-450 is active in most or all tissues of the early mammalian embryo. It could metabolize xenobiotic molecules reaching the conceptus near the onset of morphogenesis and organogenesis, or it could have another as yet undefined role in normal development.