A visual thalamocortical slice
A visual thalamocortical slice
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DOI：
10.1038/nmeth849
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发表时间：
2006-02-01
期刊：
影响因子：
48
通讯作者：
Yuste, R
中科院分区：
文献类型：
--
作者：
MacLean, JN;Fenstermaker, V;Yuste, R
我们描述了一种丘脑皮质切片制备方法，其中保留了小鼠外侧膝状核(LGN)和初级视觉皮质(V1)之间的连接。通过在固定的大脑中注射Di1，我们追踪并创建了小鼠视觉通路的三维模型。根据这个计算机模型，我们设计了一个包含从LGN到V1的投影的切片制备。我们准备了具有这些预测坐标的脑片，并在注射LGN示踪剂后在这些切片中展示了解剖上的LGN-V1连接。我们还通过电刺激LGN和使用钙成像、场电位记录和全细胞记录检测V1层的反应来揭示LGN-V1的功能连接。我们还确定了接受丘脑皮质直接输入的第四层神经元。最后，我们比较了刺激LGN后的皮质活动和自发的皮质活动，发现两种类型的事件产生的时空动力学显著重叠。
We describe a thalamocortical slice preparation in which connectivity between the mouse lateral geniculate nucleus (LGN) and primary visual cortex (V1) is preserved. Through Di1 injections in fixed brains we traced and created a three-dimensional model of the mouse visual pathways. From this computer model we designed a slice preparation that contains a projection from LGN to V1. We prepared brain slices with these predicted coordinates and demonstrated anatomical LGN-V1 connectivity in these slices after LGN tracer injections. We also revealed functional LGN-V1 connectivity by stimulating LGN electrically and detecting responses in layer 4 of V1 using calcium imaging, field potential recordings and whole-cell recordings. We also identified layer-4 neurons that receive direct thalamocortical input. Finally, we compared cortical activity after LGN stimulation with spontaneous cortical activity and found significant overlap of the spatiotemporal dynamics generated by both types of events.