Two-stage elevation of cell-free fetal DNA in maternal sera before onset of preeclampsia
Two-stage elevation of cell-free fetal DNA in maternal sera before onset of preeclampsia
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DOI：
10.1016/j.ajog.2003.12.019
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发表时间：
2004-03-01
影响因子：
9.8
通讯作者：
Bianchi, DW
中科院分区：
文献类型：
--
作者：
Levine, RJ;Qian, C;Bianchi, DW
目的：目的是为了确定是否先兆子痫（PE）是由合胞滋养层脱落到母体循环，刺激夸张的炎症respons.Study设计：嵌套病例对照研究内进行的钙对先兆子痫预防试验队列的健康初产妇的微片段。每例先兆子痫病例均与1例血压正常的对照组相匹配。随机选择120对样本，分析临产前获得的所有658份样本中的血清无细胞胎儿DNA（cffDNA）（胎盘碎片标志物）和C反应蛋白（CRP）（炎症标志物）。结果：在妊娠29至41周时，先兆子痫后cffDNA浓度显着高于之前（219 vs 112基因组当量[GE]/mL，P
Objective: The purpose was to determine Whether preeclampsia (PE) is caused by microfragments of syncytial trophoblast shed into the maternal circulation that stimulate an exaggerated inflammatory response.Study design: A nested case control study was performed within the Calcium for Preeclampsia Prevention trial cohort of healthy nulliparous women. Each preeclampsia case was matched to I normotensive control. One hundred twenty pairs were randomly chosen for analysis of serum cell-free fetal DNA (cffDNA), a marker of placental debris, and C-reactive protein (CRP), a marker of inflammation, in all 658 specimens obtained before labor.Results: At 29 to 41 weeks of gestation, cffDNA concentrations were significantly higher after preeclampsia than before (219 vs 112 genome equivalents [GE]/mL, P