Depot-specific regulation of autotaxin with obesity in human adipose tissue
Depot-specific regulation of autotaxin with obesity in human adipose tissue
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DOI：
10.1007/s13105-012-0181-z
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发表时间：
2012-12-01
影响因子：
3.4
通讯作者：
Saulnier-Blache, Jean Sebastien
中科院分区：
文献类型：
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作者：
Rancoule, Chloe;Dusaulcy, Rodolphe;Saulnier-Blache, Jean Sebastien
自体趋化蛋白(ATX)是一种溶血磷脂酶D，参与合成一种生物活性介质：溶血磷脂。ATX由脂肪细胞大量产生，对脂肪组织的扩张有负面作用。在小鼠和人类中，ATX的表达随着肥胖和胰岛素抵抗的增加而增加。在本研究中，我们探索了人类体内脂肪仓库对ATX的特异性调节。对肥胖组(BMI+lt；40 kg/m(2)；n=27)和非肥胖组(BMI+lt；25 kg/m(2)；n=10)内脏和皮下脂肪组织中ATX mRNA的表达进行了定量检测。无论患者的体重状况如何，ATX在皮下的表达总是高于内脏脂肪(1.3-6倍)。然而，肥胖者内脏脂肪ATX显著高于非肥胖者(42%)，而皮下脂肪ATX不变。肥胖组内脏脂肪ATx表达与舒张压呈正相关(r=0.67，P=0.001)。这种相关性在皮下脂肪ATX中没有观察到。内脏脂肪ATx主要与瘦素(r=0.6；P=0.001)、诱导型一氧化氮合酶(r=0.58；P=0.007)、apelin受体(r=0.5；P=0.007)相关。皮下脂肪ATX没有观察到这些相关性。这些结果表明，肥胖相关的人类脂肪组织ATX的上调是内脏脂肪库特有的。
Autotaxin (ATX) is a lysophospholipase D involved in synthesis of a bioactive mediator: lysophosphatidic. ATX is abundantly produced by adipocytes and exerts a negative action on adipose tissue expansion. In both mice and humans, ATX expression increases with obesity in association with insulin resistance. In the present study, fat depot-specific regulation of ATX was explored in human. ATX mRNA expression was quantified in visceral and subcutaneous adipose tissue in obese (BMI > 40 kg/m(2); n = 27) and non-obese patients (BMI < 25 kg/m(2); n = 10). Whatever the weight status of the patients is, ATX expression was always higher (1.3- to 6-fold) in subcutaneous than in visceral fat. Nevertheless, visceral fat ATX was significantly higher (42 %) in obese than in non-obese patients, whereas subcutaneous fat ATX remained unchanged. In obese patients, visceral fat ATX expression was positively correlated with diastolic arterial blood pressure (r = 0.67; P = 0.001). This correlation was not observed with subcutaneous fat ATX. Visceral fat ATX was mainly correlated with leptin (r = 0.60; P = 0.001), inducible nitric oxide synthase (r = 0.58; P = 0,007), and apelin receptor (r = 0.50; P = 0.007). These correlations were not observed with subcutaneous fat ATX. These results reveal that obesity-associated upregulation of human adipose tissue ATX is specific to the visceral fat depot.