In situ hybridization reveals differential spatial distribution of mRNAs for type I and type II collagen in the chick limb bud.
In situ hybridization reveals differential spatial distribution of mRNAs for type I and type II collagen in the chick limb bud.
复制标题
原位杂交揭示了鸡肢芽中 I 型和 II 型胶原蛋白 mRNA 的差异空间分布。
作者：
C. J. Devlin;Paul M. Brickell;E. R. Taylor;A. Hornbruch;R. Craig;Lewis Wolpert
在肢体发育期间，I型胶原从软骨发育的区域消失，并且开始合成作为软骨特征的II型胶原。使用反义RNA探针的原位杂交被用来研究I型和II型胶原mRNA的空间定位。II型胶原的mRNA的分布与II型胶原合成的模式相对应，这表明在转录和mRNA积累水平上的控制。相比之下，I型胶原蛋白的mRNA的模式保持或多或少的均匀性，并不对应于蛋白质的合成，这表明控制主要在翻译或RNA加工的水平。
During limb development, type I collagen disappears from the region where cartilage develops and synthesis of type II collagen, which is characteristic of cartilage, begins. In situ hybridization using antisense RNA probes was used to investigate the spatial localization of type I and type II collagen mRNAs. The distribution of the mRNA for type II collagen corresponded well with the pattern of type II collagen synthesis, suggesting control at the level of transcription and mRNA accumulation. In contrast, the pattern of mRNA for type I collagen remained more or less uniform and did not correspond with the synthesis of the protein, suggesting control primarily at the level of translation or of RNA processing.
影响因子：
2.7
作者：
KRAVIS, D;UPHOLT, WB
通讯作者：
UPHOLT, WB