Microfabricated structures for integrated DNA analysis
Microfabricated structures for integrated DNA analysis
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DOI：
10.1073/pnas.93.11.5556
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发表时间：
1996-05-28
影响因子：
11.1
通讯作者：
Burke, DT
中科院分区：
文献类型：
--
作者：
Burns, MA;Mastrangelo, CH;Burke, DT
硅的光刻微加工是构建高通量DNA分析设备的候选技术。然而，复杂硅微加工系统的发展一直受到阻碍，部分原因是缺乏一种简单、通用的泵送方法来集成单个组件。在这里，我们描述了一种基于表面张力的泵，它能够通过封闭的通道移动离散的纳升液滴，仅使用局部加热。这种热毛细管泵可以准确地混合，测量，并通过简单的电子控制分滴。此外，我们已经构建了热循环室，凝胶电泳通道和放射性标记的DNA探测器，它们与热毛细管泵通道的制造兼容。由于所有组件都是由传统的光刻技术制造的，因此它们可以组装成更复杂的集成系统。将泵和组件组合成独立的小型化设备，可以显著提高DNA分析速度、便携性和成本。微加工系统的潜力在于硅基结构的低单位成本和组件集成提供的有效样品处理。
Photolithographic micromachining of silicon is a candidate technology for the construction of high-throughput DNA analysis devices. However, the development of complex silicon microfabricated systems has been hindered in part by the lack of a simple, versatile pumping method for integrating individual components. Here we describe a surface-tension-based pump able to move discrete nanoliter drops through enclosed channels using only local heating. This thermocapillary pump can accurately mix, measure, and divide drops by simple electronic control. In addition, we have constructed thermal-cycling chambers, gel electrophoresis channels, and radiolabeled DNA detectors that are compatible with the fabrication of thermocapillary pump channels. Since all of the components are made by conventional photolithographic techniques, they can be assembled into more complex integrated systems. The combination of pump and components into self-contained miniaturized devices may provide significant improvements in DNA analysis speed, portability, and cost. The potential of microfabricated systems lies in the low unit cost of silicon-based construction and in the efficient sample handling afforded by component integration.