CLONALITY, EXPRESSION AND METHYLATION PATTERNS OF THE EPSTEIN-BARR-VIRUS GENOMES IN LETHAL MIDLINE GRANULOMAS CLASSIFIED AS PERIPHERAL ANGIOCENTRIC T-CELL LYMPHOMAS
CLONALITY, EXPRESSION AND METHYLATION PATTERNS OF THE EPSTEIN-BARR-VIRUS GENOMES IN LETHAL MIDLINE GRANULOMAS CLASSIFIED AS PERIPHERAL ANGIOCENTRIC T-CELL LYMPHOMAS
复制标题
DOI：
10.1099/0022-1317-75-1-77
复制
发表时间：
1994-01-01
影响因子：
3.8
通讯作者：
KLEIN, G
中科院分区：
文献类型：
--
作者：
MINAROVITS, J;HU, LF;KLEIN, G
我们分析了EB病毒（EBV）的末端重复DNAs分离自6个致命的中线肉芽肿（LMG）活检。在每种情况下，可以检测到一个单一的融合的末端片段，表明这些血管中心性外周T细胞淋巴瘤代表在一个单一的场合与EBV感染的细胞的克隆性增殖。逆转录聚合酶链反应（RT-PCR）检测LMG活检RNA中EB病毒核抗原（EBNA）1和潜伏膜蛋白（LMP）1，但未检测到EBNA 2。EBNA 1信息的剪接模式与定位于BamHI F片段中的启动子（F启动子）的使用一致。在所有病例中，BamHI W片段重复序列和LMP编码序列高度甲基化。相反，LMP调控序列被发现是低甲基化或部分甲基化，如在LMP表达鼻咽癌。
We analysed the terminal repeats of Epstein-Barr virus (EBV) in DNAs isolated from six lethal midline granuloma (LMG) biopsies. A single fused terminal fragment could be detected in each case, indicating that these angiocentric peripheral T cell lymphomas represent clonal proliferations of cells infected with EBV on a single occasion. Using reverse transcriptase-PCR, we detected EBV nuclear antigen (EBNA) 1 and latent membrane protein (LMP) 1, but not EBNA 2 messages in LMG biopsy RNAs. The splicing pattern of the EBNA 1 message was consistent with the usage of a promoter localized in the BamHI F fragment (F promoter). The BamHI W fragment repeats and LMP-coding sequences were highly methylated in ah cases. In contrast, the LMP regulatory sequences were found to be hypomethylated or partially methylated, as in LMP-expressing nasopharyngeal carcinomas.