DNA STRUCTURAL VARIATIONS IN THE ESCHERICHIA-COLI TYRT-PROMOTER
DNA STRUCTURAL VARIATIONS IN THE ESCHERICHIA-COLI TYRT-PROMOTER
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DOI：
10.1016/0092-8674(84)90379-9
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发表时间：
1984-01-01
期刊：
影响因子：
64.5
通讯作者：
TRAVERS, AA
中科院分区：
文献类型：
--
作者：
DREW, HR;TRAVERS, AA
X射线研究证实，一个右手的沃森-克里克双螺旋的结构可以随着碱基序列的变化而沿着其长度从一个地方到另一个地方发生变化。碱基对将变形传递到磷酸骨架，然后它们可以被蛋白质和其他DNA结合试剂识别。3种常用的核酸酶（DNAase I，DNAase II和Cu-菲咯啉）与天然来源的DNA，160个碱基对的tyrT启动子的相互作用进行了检查，在单键分辨率。所有这3种试剂似乎对DNA骨架几何形状而不是碱基序列本身敏感。它们的序列依赖型裂解提供了几种结构多态性的证据：螺旋槽宽度的全球变化，径向不对称性的全球变化和磷酸盐可及性的局部变化。这些发现解释了特定碱基组成的序列区以及嘌呤-嘧啶不对称性如何影响蛋白质分子对DNA的识别。
X-ray studies established that the structure of a right-handed, Watson-Crick double helix can change from place to place along its length as a function of base sequence. The base pairs transmit deformations out to the phosphate backbone, where they can then be recognized by proteins and other DNA-binding reagents. The interactions of 3 commonly used nucleases (DNAase I, DNAase II and Cu-phenanthroline) with a DNA of natural origin, the 160 base pair tyrT promoter were examined at single-bond resolution. All 3 of these reagents seem sensitive to DNA backbone geometry rather than base sequence per se. Their sequence-dependent patterns of cleavage provide evidence for structural polymorphism of several sorts: global variation in helix groove width, global variation in radial asymmetry and local variation in phosphate accessibility. These findings explain how sequence zones of a certain base composition, and purine-pyrimidine asymmetry, can influence the recognition of DNA by protein molecules.