Phe-Gly-Leu-amide allatostatin in the termite Reticulitermes flavipes: content in brain and corpus allatum and effect on juvenile hormone synthesis.
Phe-Gly-Leu-amide allatostatin in the termite Reticulitermes flavipes: content in brain and corpus allatum and effect on juvenile hormone synthesis.
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DOI：
10.1016/j.jinsphys.2004.12.006
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发表时间：
2005-04
影响因子：
2.2
通讯作者：
Koichiro J. Yagi;R. Kwok;K. Chan;R. R. Setter-R.;Timothy G. Myles;S. Tobe;B. Stay
中科院分区：
文献类型：
--
作者：
Koichiro J. Yagi;R. Kwok;K. Chan;R. R. Setter-R.;Timothy G. Myles;S. Tobe;B. Stay
用免疫细胞化学方法，用抗蟑螂AST的抗体Dippu AST-7，在地下白蚁黄胸散白蚁体内定位了C-末端为Phe-Gly亮氨酸氨基的烯类生长抑素(AST)。AST免疫反应阳性反应见于心体和扁桃体，以及支配这些器官的大脑的外侧和内侧神经分泌细胞，以及大脑的许多其他神经细胞。这在工蜂、若虫、士兵和二次繁殖者身上都能观察到。使用抗Dippu AST-11的放射免疫分析表明，士兵和二次繁殖者的大脑中AST的当量约为40fmole。经体外放射化学测定，该产物为保幼激素III。两种蟑螂降钙素DIPPU AST-7和DIPPU AST-11以剂量依赖的方式抑制二次繁殖体的合成。因此，就像蟑螂和蟋蟀一样，含有生长抑素的神经支配着这种白蚁物种的翼体，它们的保幼激素的产生被这些神经肽抑制。
In the subterranean termite Reticulitermes flavipes, allatostatins (ASTs) with the C-terminus Phe-Gly Leu-amide were localized by immunocytochemistry with antibody against a cockroach AST, Dippu AST-7. AST-immunoreactivity occurred in the corpus cardiacum and corpus allatum and in the lateral and medial neurosecretory cells of the brain that innervate these organs as well as in many other nerve cells of the brain. This was observed in workers, nymphs, soldiers and secondary reproductives. A radioimmunoassay, using anti-Dippu AST-11, demonstrated about 40fmole equivalents of AST in brains of soldiers and secondary reproductives. The product of the corpora allata in this species was determined to be juvenile hormone III. Its synthesis by corpora allata of secondary reproductives, determined by in vitro radiochemical assay, was inhibited in a dose-dependent fashion by two cockroach allatostatins, Dippu AST-7 and Dippu AST-11. Thus, as in cockroaches and crickets, allatostatin-containing nerves innervate the corpora allata of this termite species and their production of juvenile hormone is inhibited by these neuropeptides.