Mass Spectrometric Analysis of Proteins

Mass Spectrometric Analysis of Proteins
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DOI：
10.1007/978-1-4939-6887-9_25
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发表时间：
2017-01-01
期刊：
HETEROLOGOUS GENE EXPRESSION IN E. COLI: METHODS AND PROTOCOLS
影响因子：
--
通讯作者：
Chalk, Rod
Chalk, Rod
中科院分区：
其他
文献类型：
--
作者：
Chalk, Rod

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质谱法是一种用于纯化蛋白质结构和功能分析的通用技术。讨论了仪器性能和完整蛋白、肽片段和原生分析的可能性。详细的实验方案描述了最常用的蛋白质鉴定技术，翻译后修饰（PTM）表征，PTM制图，天然质谱分析，并使用电喷雾质谱分析膜蛋白。
Mass spectrometry is a generic technique for the structural and functional analysis of purified proteins. Instrument capabilities and the possibilities of intact protein, peptide fragmentation and native analyses are discussed. Detailed experimental protocols are described for the most commonly applied techniques of protein identification, posttranslational modification (PTM) characterization, PTM mapping, native mass spectrometry, and analysis of membrane proteins using electrospray mass spectrometry.