IMMUNOCYTOCHEMICAL LOCALIZATION OF 5-ALPHA-REDUCTASE IN RAT-BRAIN
IMMUNOCYTOCHEMICAL LOCALIZATION OF 5-ALPHA-REDUCTASE IN RAT-BRAIN
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DOI：
10.1006/mcne.1994.1049
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发表时间：
1994-10-01
影响因子：
3.5
通讯作者：
LABRIE, F
中科院分区：
文献类型：
--
作者：
PELLETIER, G;VAN, LT;LABRIE, F
脑组织中类固醇5 α-还原酶的存在是有据可查的。为了精确地确定成年大鼠中含有这种酶的脑区和神经细胞类型，我们使用包含该酶的氨基酸227至240的序列肽的抗体研究了其免疫细胞化学定位。在光镜水平，5 α-还原酶免疫反应性物质似乎只存在于神经胶质和室管膜细胞，包括伸长细胞。在下丘脑、丘脑、海马、大脑皮质、大脑皮质和脑室周围器官中发现高浓度的免疫标记细胞。免疫电镜研究表明，免疫反应性物质弥漫分布在整个胶质细胞和室管膜细胞的细胞质中，从而证实了5 α-还原酶免疫反应性与非神经元细胞的关联。这些数据表明，在成年大鼠体内条件下，胶质细胞在睾酮转化为双氢睾酮或孕酮转化为双氢孕酮中起重要作用。(C)1994年出版社出版。
The presence of steroid 5 alpha-reductase in brain tissue is well-documented. In order to determine with precision the brain areas and nerve cell types containing this enzyme in adult rat, we have studied its immunocytochemical localization using antibodies to the sequence peptide comprising amino acids 227 to 240 of the enzyme. At the light microscopic level, 5 alpha-reductase immunoreactive material appeared to be present only in glial and ependymal cells, including tanycytes. Immunolabeled cells were found in high concentration in the hypothalamus, thalamus, hippocampus, cerebral cortex, cerebral cortex, and circumventricular organs. Immunoelectron microscopic studies demonstrated that immunoreactive material was diffusely distributed throughout the cytoplasm of glial and ependymal cells, thus confirming the association of 5 alpha-reductase immunoreactivity with nonneuronal cells. These data suggest that glial cells play an important role in the conversion of testosterone to dihydrotestosterone or progesterone to dihydroprogesterone under in vivo conditions in adult rat. (C) 1994 Academic Press, Inc.