Human thymic stromal lymphopoietin preferentially stimulates myeloid cells
Human thymic stromal lymphopoietin preferentially stimulates myeloid cells
复制标题
DOI：
10.4049/jimmunol.167.1.336
复制
发表时间：
2001-07-01
影响因子：
4.4
通讯作者：
Bazan, JF
中科院分区：
文献类型：
--
作者：
Reche, PA;Soumelis, V;Bazan, JF
在基因组数据库的计算筛选中发现了一种新的造血细胞因子的序列，其与小鼠胸腺基质淋巴细胞生成素（TSLP）的同源性表明它是人类的直系同源物。提出人TSLP通过异源二聚体受体复合物发出信号，所述异源二聚体受体复合物由称为人TSLP受体的促红细胞生成素家族的新成员和IL-7 R α链组成。两种受体亚基转染的细胞增殖响应纯化的重组人TSLP，诱导磷酸化的Stat 3和Stat 5。人TSLPR和IL-7 Ra主要在单核细胞和树突细胞群上共表达，在各种淋巴样细胞上共表达的程度要小得多。与此雅阁，我们发现人TSLP主要作用于髓系细胞;它诱导单核细胞释放T细胞吸引趋化因子，特别是增强CD 11 c（+）树突状细胞的成熟，如通过强烈诱导共刺激分子CD 40和CD 80以及增强诱导幼稚T细胞增殖的能力所证明的。
The sequence of a novel hemopoietic cytokine was discovered in a computational screen of genomic databases, and its homology to mouse thymic stromal lymphopoietin (TSLP) suggests that it is the human orthologue. Human TSLP is proposed to signal through a heterodimeric receptor complex that consists of a new member of the hemopoietin family termed human TSLP receptor and the IL-7R a-chain. Cells transfected with both receptor subunits proliferated in response to purified, recombinant human TSLP, with induced phosphorylation of Stat3 and Stat5. Human TSLPR and IL-7R alpha are principally coexpressed on monocytes and dendritic cell populations and to a much lesser extent on various lymphoid cells. In accord, we find that human TSLP functions mainly on myeloid cells; it induces the release of T cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells, as evidenced by the strong induction of the costimulatory molecules CD40 and CD80 and the enhanced capacity to elicit proliferation of naive T cells.