Evaluation of an in vitro muscle contraction model in mouse primary cultured myotubes
Evaluation of an in vitro muscle contraction model in mouse primary cultured myotubes
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DOI：
10.1016/j.ab.2015.10.010
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发表时间：
2016-03-15
影响因子：
2.9
通讯作者：
Fujii, Nobuharu L.
中科院分区：
文献类型：
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作者：
Manabe, Yasuko;Ogino, Shinya;Fujii, Nobuharu L.
为了建立小鼠指长伸肌的体外收缩模型，我们从小鼠指长伸肌中分离卫星细胞。与C2C12肌管相比，分化后的肌管具有更多的肌节组装体和更高的肌球蛋白重链、细胞色素c氧化酶IV和肌红蛋白表达水平。与这些关于肌节组装和蛋白质表达的结果一致，原发肌管在电脉冲刺激下表现出比C2C12肌管更高的收缩活性。这些数据表明，小鼠原代肌管作为体外肌肉收缩模型将是一个有价值的研究工具。(C) 2015爱思唯尔公司版权所有。
To construct an in vitro contraction model with the primary cultured myotubes, we isolated satellite cells from the mouse extensor digitorum longus. Differentiated myotubes possessed a greater number of sarcomere assemblies and higher expression levels of myosin heavy chain, cytochrome c oxidase IV, and myoglobin than in C2C12 myotubes. In agreement with these results regarding the sarcomere assemblies and protein expressions, the primary myotubes showed higher contractile activity stimulated by the electric pulses than that in the C2C12 myotubes. These data suggest that mouse primary myotubes will be a valuable research tool as an in vitro muscle contraction model. (C) 2015 Elsevier Inc. All rights reserved.