Epidemiology, Genetic Characterization, and Evolution of Hunnivirus Carried by Rattus norvegicus and Rattus tanezumi: The First Epidemiological Evidence from Southern China
Epidemiology, Genetic Characterization, and Evolution of Hunnivirus Carried by Rattus norvegicus and Rattus tanezumi: The First Epidemiological Evidence from Southern China
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DOI：
10.3390/pathogens10060661
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发表时间：
2021-06-01
期刊：
影响因子：
3.7
通讯作者：
Chen, Qing
中科院分区：
文献类型：
--
作者：
Zhang, Minyi;Li, Qiushuang;Chen, Qing
猎人病毒是小核糖核酸病毒科的新成员。目前描述了单一物种，Hunnivirus A。然而，迄今为止，有关Hunnivirus的鉴定信息有限，因此Hunnivirus的传播情况尚不完全清楚。因此，本研究的目的是调查中国南方地区大鼠兔病毒的流行情况、基因组特征和进化。采用针对部分3D区域的特异性引物进行PCR检测，对404份城市大鼠（褐家鼠和褐家鼠）粪便样本进行了Hunnivirus的检测，其中褐家鼠和褐家鼠的检出率为17.8%，褐家鼠为15.6%。本研究获得了几乎全长的大鼠猎人病毒序列（RatHuV/YY12/CHN）和基因组结构。 P1 编码区的系统发育分析表明 RatHuV/YY12/CHN 序列位于 Hunnivirus A4 的基因型内。根据非同义替换率的分析进一步确定了负选择。目前的研究结果表明，人类病毒在城市老鼠中很常见。需要进一步研究以加强对人类健康潜在风险的监测和认识。
Hunnivirus is a novel member of the family Picornaviridae. A single species, Hunnivirus A, is currently described. However, there is limited information on the identification of Hunnivirus to date, and thereby the circulation of Hunnivirus is not fully understood. Thus, the objective of this study was to investigate the prevalence, genomic characteristics, and evolution of rat hunnivirus in southern China. A total of 404 fecal samples were subjected to detection of Hunnivirus from urban rats (Rattus norvegicus and Rattus tanezumi) using PCR assay based on specific primers targeted to partial 3D regions, with the prevalence of 17.8% in Rattus norvegicus and 15.6% in Rattus tanezumi. An almost full-length rat hunnivirus sequence (RatHuV/YY12/CHN) and the genome structure were acquired in the present study. Phylogenetic analysis of the P1 coding regions suggested the RatHuV/YY12/CHN sequence was found to be within the genotype of Hunnivirus A4. The negative selection was further identified based on analysis of non-synonymous to synonymous substitution rates. The present findings suggest that hunniviruses are common in urban rats. Further research is needed for increased surveillance and awareness of potential risks to human health.