Fabrication and characterization of hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels for bone tissue engineering
Fabrication and characterization of hydroxypropyl guar-poly (vinyl alcohol)-nano hydroxyapatite composite hydrogels for bone tissue engineering
复制标题
DOI：
10.1080/09205063.2018.1494437
复制
发表时间：
2018-01-01
影响因子：
3.6
通讯作者：
Ghosh, Anindya
中科院分区：
文献类型：
--
作者：
Parameswaran-Thankam, Anil;Al-Anbaky, Qudes;Ghosh, Anindya
由天然材料组成的生物相容性骨植入物在骨科重建过程中是非常理想的。本研究以羟丙基瓜尔胶(HPG)、聚乙烯醇(PVA)和纳米羟基磷灰石(n-HA)为原料，在温和的反应条件下，通过冻融反应合成了新型、环保的纳米羟基磷灰石复合水凝胶。用各种技术对水凝胶材料进行了表征。热重分析表明，HPG/PVA和HPG/PVA/n-HA复合材料的热稳定性均高于HPG/PVA/n-HA，而HPG/PVA/n-HA复合材料的热稳定性高于PVA。扫描电子显微镜显示，HPG/PVA水凝胶呈多孔状，适合骨组织再生。此外，水凝胶被发现是透明和灵活的性质。在模拟体液中进行的体外生物矿化研究表明，HPG/PVA/n-HA具有类磷灰石结构。将该水凝胶材料作为细胞外基质进行生物相容性研究。采用四甲基偶氮唑蓝、台盼蓝拒染、溴化乙锭/吖啶橙染色等方法对体外培养的小鼠成骨细胞MC3T3进行细胞活性检测。细胞活性研究表明，与原始的PVA相比，复合材料支持细胞生长，没有任何细胞毒性的迹象。在复合水凝胶基质上生长的MC3T3骨细胞的碱性磷酸酶活性增加，证实了成骨活性。
Biocompatible bone implants composed of natural materials are highly desirable in orthopedic reconstruction procedures. In this study, novel and ecofriendly bionanocomposite hydrogels were synthesized using a blend of hydroxypropyl guar (HPG), poly vinyl alcohol (PVA), and nano-hydroxyapatite (n-HA) under freeze-thaw and mild reaction conditions. The hydrogel materials were characterized using various techniques. TGA studies indicate that both composites, HPG/PVA and HPG/PVA/n-HA, have higher thermal stability compared to HPG alone whereas HPG/PVA/n-HA shows higher stability compared to PVA alone. The HPG/PVA hydrogel shows porous morphology as revealed by the SEM, which is suitable for bone tissue regeneration. Additionally, the hydrogels were found to be transparent and flexible in nature. In vitro biomineralization study performed in simulated body fluid shows HPG/PVA/n-HA has an apatite like structure. The hydrogel materials were employed as extracellular matrices for biocompatibility studies. In vitro cell viability studies using mouse osteoblast MC3T3 cells were performed by MTT, Trypan blue exclusion, and ethidium bromide/acridine orange staining methods. The cell viability studies reveal that composite materials support cell growth and do not show any signs of cytotoxicity compared to pristine PVA. Osteoblastic activity was confirmed by an increased alkaline phosphatase enzyme activity in MC3T3 bone cells grown on composite hydrogel matrices.