dbMAE: the database of autosomal monoallelic expression.
dbMAE: the database of autosomal monoallelic expression.
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DOI：
10.1093/nar/gkv1106
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发表时间：
2016-01-04
影响因子：
14.9
通讯作者：
Gimelbrant AA
中科院分区：
文献类型：
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作者：
Savova V;Patsenker J;Vigneau S;Gimelbrant AA
最近，关于“随机”常染色体单等位基因表达的数据已可用于多种人类和小鼠组织和细胞类型中的整个基因组，从而需要更好地获取和传播。常染色体单等位基因表达数据库（dbMAE; https://mae.hms.harvard.edu）整合了来自多个最近全基因组分析报告的数据。这些包括基于序列多态性的克隆细胞群体中等位基因不平衡的全转录组分析，以及基于MAE基因体中存在的特定染色质特征的间接鉴定。目前，dbMAE包含8种人类和21种小鼠组织的全转录组染色质鉴定调用，并描述了超过16000例小鼠和10700例人类直接测量的偏倚表达病例，这些病例是根据等位基因特异性RNA-seq和基因分型阵列数据汇编的。所有数据都是手动管理的。为了确保交叉发表的一致性，我们使用相同的管道对转录组范围的RNA-seq数据进行了重新分析。通过一个允许基本和高级搜索的界面访问数据;所有参考来源，包括原始数据，都有明确的说明和超链接。这确保了该资源作为那些有兴趣研究单等位基因表达在其特定基因和组织中的作用的人的初始筛选工具的效用。
Recently, data on ‘random’ autosomal monoallelic expression has become available for the entire genome in multiple human and mouse tissues and cell types, creating a need for better access and dissemination. The database of autosomal monoallelic expression (dbMAE; https://mae.hms.harvard.edu) incorporates data from multiple recent reports of genome-wide analyses. These include transcriptome-wide analyses of allelic imbalance in clonal cell populations based on sequence polymorphisms, as well as indirect identification, based on a specific chromatin signature present in MAE gene bodies. Currently, dbMAE contains transcriptome-wide chromatin identification calls for 8 human and 21 mouse tissues, and describes over 16 000 murine and ∼700 human cases of directly measured biased expression, compiled from allele-specific RNA-seq and genotyping array data. All data are manually curated. To ensure cross-publication uniformity, we performed re-analysis of transcriptome-wide RNA-seq data using the same pipeline. Data are accessed through an interface that allows for basic and advanced searches; all source references, including raw data, are clearly described and hyperlinked. This ensures the utility of the resource as an initial screening tool for those interested in investigating the role of monoallelic expression in their specific genes and tissues of interest.