The genomic structure of the human Charcot-Leyden crystal protein gene is analogous to those of the galectin genes
The genomic structure of the human Charcot-Leyden crystal protein gene is analogous to those of the galectin genes
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DOI：
10.1006/geno.1996.4590
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发表时间：
1997-03-01
期刊：
影响因子：
4.4
通讯作者：
Rosenberg, HF
中科院分区：
文献类型：
--
作者：
Dyer, KD;Handen, JS;Rosenberg, HF
Charcot-Leyden晶体（CLC）蛋白或嗜酸性粒细胞溶血磷脂酶是人类嗜酸性粒细胞和嗜碱性粒细胞的特征蛋白;最近的研究表明，CLC蛋白在结构和功能上都与β-半乳糖苷结合蛋白的半乳糖凝集素家族相关。半乳糖凝集素作为一个组共享一些共同的特征，包括在单个外显子上编码的线性配体结合位点。在这项工作中，我们证明了编码CLC的基因的内含子-外显子结构类似于编码半乳糖凝集素的那些。CLC基因的编码序列分为四个外显子，其中整个β-半乳糖苷结合位点由外显子III编码。我们已经从猩猩（Pongo pygmaeus）和小鼠（Mus musculus）基因组DNA中分离出CLC β-半乳糖苷结合位点，这两种基因组DNA都编码在单个外显子上，并注意到与β-半乳糖苷配体直接相互作用的氨基酸的保守性。这些结果的最可能的解释表明发生一个或多个外显子重复和插入事件，导致该凝集素结构域分布到CLC以及多个半乳糖凝集素基因。
The Charcot-Leyden crystal (CLC) protein, or eosinophil lysophospholipase, is a characteristic protein of human eosinophils and basophils; recent work has demonstrated that the CLC protein is both structurally and functionally related to the galectin family of beta-galactoside binding proteins. The galectins as a group share a number of features in common, including a linear ligand binding site encoded on a single exon, In this work, we demonstrate that the intron-exon structure of the gene encoding CLC is analogous to those encoding the galectins. The coding sequence of the CLC gene is divided into four exons, with the entire beta-galactoside binding site encoded by exon III. We have isolated CLC beta-galactoside binding sites from both orangutan (Pongo pygmaeus) and murine (Mus musculus) genomic DNAs, both encoded on single exons, and noted conservation of the amino acids shown to interact directly with the beta-galactoside ligand. The most likely interpretation of these results suggests the occurrence of one or more exon duplication and insertion events, resulting in the distribution of this lectin domain to CLC as well as to the multiple galectin genes.