Guided tissue fabrication from periosteum using preformed biodegradable polymer scaffolds
Guided tissue fabrication from periosteum using preformed biodegradable polymer scaffolds
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DOI：
10.1016/s0142-9612(99)00103-9
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发表时间：
1999-11-01
期刊：
影响因子：
14
通讯作者：
Miller, MJ
中科院分区：
文献类型：
--
作者：
Thomson, RC;Mikos, AG;Miller, MJ
成功的骨替代组织工程方法将通过使用合成支架、成骨细胞群和骨诱导因子提供新骨形成的基本要素来模仿天然骨移植。这是一项研究聚（DL-乳酸-乙醇酸）（PLGA）泡沫的各种配方在绵羊模型中为骨瓣制造提供组织传导支架的适用性。使用不同共聚物比例和分子量的三种配方。将 PLGA 多孔晶片堆叠到五个侧面封闭的矩形室（体积 4 cm(3)）中。一些腔室还含有自体粉碎骨移植物（MBG）。将室插入七只羊的肋骨床中，其开放面邻近骨膜形成层，并在体内8周后收获。对所得组织样本的大体和组织学检查表明，血管化组织的模制单元通常符合腔室的形状并牢固地附着在骨膜上。根据聚合物配方，聚合物降解似乎发生不同程度。仅在含有 MEG 的区域观察到新骨形成。 8周时没有明显炎症反应或局部组织损伤的证据。我们得出的结论是，PLGA 泡沫支架 (1) 是新组织生长的有效导体，但不具有骨诱导性，(2) 有助于成型组织的形状，(3) 在该模型中使用时具有生物相容性。需要进一步的研究来开发实用的方法，将骨诱导因子传递到系统中，以促进整个合成支架中骨组织的生成。 (C) 1999 Elsevier Science Ltd. 保留所有权利。
A successful tissue engineering method for bone replacement would imitate natural bone graft by providing the essential elements for new bone formation using synthetic scaffolds, osteogenic cell populations, and bone induction factors. This is a study of the suitability of various formulations of poly(DL-lactic-co-glycolic acid) (PLGA) foams to provide a tissue conducting scaffold in an ovine model for bone flap fabrication. Three formulations were used of different copolymer ratio and molecular weight. Porous wafers of PLGA were stacked into rectangular chambers (volume 4 cm(3)) enclosed on five sides. Some chambers also contained autologous morcellized bone graft (MBG). The chambers were inserted with the open face adjacent to the cambium layer of the periosteum in rib beds of seven sheep and harvested after 8 weeks in vivo. Gross and histologic examination of the resulting tissue specimens demonstrated molded units of vascularized tissue generally conforming to the shape of the chambers and firmly attached to the periosteum. Polymer degradation appeared to occur by varying degrees based on polymer formulation. New bone formation was observed only in areas containing MEG. There was no evidence of significant inflammatory reaction or local tissue damage at 8 weeks. We conclude that a PLGA foam scaffold is (1) an efficient conductor of new tissue growth but not osteoinductive, (2) contributes to the shape of molded tissue, and (3) biocompatible when used in this model. Further studies are warranted to develop practical methods to deliver bone induction factors to the system to promote osseous tissue generation throughout the synthetic scaffold. (C) 1999 Elsevier Science Ltd. All rights reserved.