Use of human mesenchymal stem cells as alternative source of smooth muscle cells in vessel engineering.
Use of human mesenchymal stem cells as alternative source of smooth muscle cells in vessel engineering.
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DOI：
10.1007/978-1-60761-999-4_21
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Niklason, Laura E
中科院分区：
文献类型：
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作者：
Gong, Zhaodi;Niklason, Laura E
成体干细胞来源的平滑肌细胞(SMC)可能是一种很有前途的细胞来源，可用于再生医学，包括心血管组织工程。来源于老年供者的原代血管SMC的增殖能力有限，胶原生成减少，老年供者是因冠心病或外周动脉疾病而需要血管移植的患者。我们最近的工作表明，人骨髓间充质干细胞(HMSCs)分化为SMC的能力受到各种生长因子、基质蛋白和机械力的调节。此外，培养基组分在SMC向hMSCs分化过程中起着非常重要的作用。在本章中，我们将总结各种因素对SMC向hMSCs分化的影响经验。基于我们对生长因子、循环应变和基质蛋白的研究结果，我们建立了包括4周增殖期和4周分化期的两阶段血管再生培养方案，以连续优化hMSCs的增殖和SMC分化。
Adult stem cell-derived smooth muscle cells (SMC) may be a promising source of cells for applications in regenerative medicine, including cardiovascular tissue engineering. Primary SMC from native vessels may have limited proliferative capacity and reduced collagen production when sourced from elderly donors, who are the patients in need of vascular grafts due to coronary disease or peripheral arterial disease. Our recent work showed that the ability of human bone marrow-derived mesenchymal stem cells (hMSCs) to differentiate into SMC was modulated by various growth factors, matrix proteins, and mechanical forces. In addition, the components of the culture medium play a very important role in SMC differentiation from hMSCs. In this chapter, we will summarize our experience with the impact of various factors on SMC differentiation from hMSCs. Based upon our findings regarding growth factors, cyclic strain and matrix proteins, a two-phase vessel regeneration culture protocol including a 4-week proliferation phase and a 4-week differentiation phase was developed to optimize proliferation and SMC differentiation of hMSCs consecutively.