Comparison of the immune responses in BALB/c mice following immunization with DNA-based and live attenuated vaccines delivered via different routes
Comparison of the immune responses in BALB/c mice following immunization with DNA-based and live attenuated vaccines delivered via different routes
复制标题
通过不同途径递送基于 DNA 的疫苗和减毒活疫苗免疫后 BALB/c 小鼠免疫反应的比较
DOI：
10.1016/j.vaccine.2012.09.009
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发表时间：
2013-02-18
期刊：
影响因子：
5.5
通讯作者：
Li, Mei-li
中科院分区：
文献类型：
--
作者：
Cai, Ming-sheng;Deng, Shu-xuan;Li, Mei-li
The objective of this study was to compare immune responses induced in BALB/c mice following immunization with pcDNA-GPV-VP2 DNA by gene gun bombardment (6 mu g) or by intramuscular (im) injection (100 mu g) with the responses to live attenuated vaccine by im injection (100 mu l). pcDNA3.1 (+) and physiological saline were used as controls. Peripheral blood samples were collected at 3, 7, 14, 21, 28, 35, 49, 63, 77 and 105 d after immunization. T lymphocyte proliferation was analyzed by KIT assay and enumeration of CD4(+), and CD8(+) T cell populations in peripheral blood was performed by flow cytometric analysis. Indirect ELISA was used to detect IgG levels. Cellular and humoral responses were induced by pcDNA-GPV-VP2 DNA and live virus vaccines. No differences were observed in T cell proliferation and CD8(+) T cell responses induced by the genetic vaccine regardless of the route of delivery. However, CD4(+) T cell responses and humoral immunity were enhanced in following gene gun immunization compared with im injection of the genetic vaccine. Cellular and humoral immunity was enhanced in following gene gun delivery of the genetic vaccine compared with the live attenuated vaccine. In conclusion, the pcDNA-GPV-VP2 DNA vaccine induced enhanced cellular and humoral immunity compared with that induced by the live attenuated vaccine. (C) 2012 Elsevier Ltd. All rights reserved.
