Culture of mouse giant central nervous system synapses and application for imaging and electrophysiological analyses
Culture of mouse giant central nervous system synapses and application for imaging and electrophysiological analyses
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小鼠巨型中枢神经系统突触的培养及其在成像和电生理分析中的应用
DOI：
10.1007/978-1-4939-7571-6_15
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发表时间：
2018
影响因子：
--
通讯作者：
Kohgaku Eguchi and Tomoyuki Takahashi
中科院分区：
文献类型：
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作者：
Dimitar Dimitrov;Laurent Guillaud;Kohgaku Eguchi and Tomoyuki Takahashi
原代神经元细胞培养制剂广泛用于研究突触功能。本章描述了制备神经元细胞培养物的详细方案，其中形成了巨大的花萼型突触末端。本章还介绍了利用这种制剂所提供的主要技术优势的详细协议，即直接从突触前终端进行突触细胞器的标记和成像以及电生理记录。
Primary neuronal cell culture preparations are widely used to investigate synaptic functions. This chapter describes a detailed protocol for the preparation of a neuronal cell culture in which giant calyx-type synaptic terminals are formed. This chapter also presents detailed protocols for utilizing the main technical advantages provided by such a preparation, namely, labeling and imaging of synaptic organelles and electrophysiological recordings directly from presynaptic terminals.