Dietary curcumin supplementation attenuates inflammation, hepatic injury and oxidative damage in a rat model of intra-uterine growth retardation
Dietary curcumin supplementation attenuates inflammation, hepatic injury and oxidative damage in a rat model of intra-uterine growth retardation
复制标题
膳食姜黄素补充剂可减轻子宫内生长迟缓大鼠模型的炎症、肝损伤和氧化损伤
DOI：
10.1017/s0007114518001630
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发表时间：
2018-09-14
影响因子：
3.6
通讯作者：
Wang, Tian
中科院分区：
文献类型：
--
作者：
He, Jintian;Niu, Yu;Wang, Tian
摘要正常出生体重（NBW）和宫内生长迟缓（IUGR）大鼠分别饲喂基础饲料（NBW组和IUGR组）和基础饲料中添加姜黄素（NC组和IC组）6 ~ 12周。IUGR组大鼠体重明显低于对照组（P<0.05）。IUGR组大鼠血清TNF-α、IL-1β、IL-6水平显著高于NBW组（P <0.05），AST、ALT活性显著高于NBW组（P<0.05），肝组织丙二醛（MDA）、蛋白质羰基（PC）、8-羟基脱氧鸟苷（8-OHDG）水平显著高于NBW组（P<0.05）。IUGR大鼠肝脏SOD活性和谷胱甘肽氧化还原循环代谢效率均低于NBW大鼠（P<0.05）。与IUGR组相比，IC组血清中炎性细胞因子浓度、AST和ALT活性以及肝脏中MDA、PC和8-OHDG活性均降低（P<0.05），肝脏谷胱甘肽氧化还原循环改善（P<0.05）。与IUGR大鼠相比，IC大鼠肝脏NF-κB通路和JAK 2磷酸化水平降低（P <0·05），核因子、红细胞2样蛋白2（Nfe 2l 2）/抗氧化反应元件（ARE）通路相关基因mRNA表达升高（P <0·05）。母亲营养不良会降低新生儿体重，并导致炎症、氧化损伤和大鼠损伤。姜黄素似乎通过激活肝脏中NF-κB、JAK/STAT和Nfe 212/ARE通路的表达而有益于预防IUGR诱导的炎症、氧化损伤和损伤。
Abstract Rats with a normal birth weight (NBW) or intra-uterine growth retardation (IUGR) were fed basic diets (NBW and IUGR groups) or basic diets supplemented with curcumin (NC and IC groups) from 6 to 12 weeks. The body weight of IUGR rats was lower (P<0·05) than that of the controls. Rats with IUGR showed higher (P<0·05) concentrations of TNF-α, IL-1β and IL-6; higher (P<0·05) activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in their serum; and increased (P<0·05) concentrations of malondialdehyde (MDA), protein carbonyl (PC) and 8-hydroxy-2'-deoxyguanosine (8-OHDG) in the liver compared with the NBW rats. The livers of IUGR rats exhibited a lower (P<0·05) superoxide dismutase activity and decreased (P<0·05) metabolic efficiency of the hepatic glutathione redox cycle compared with those of the NBW rats. In response to dietary curcumin supplementation, concentrations of inflammatory cytokines and activities of AST and ALT in the serum and MDA, PC and 8-OHDG in the liver were lower (P<0·05), and the hepatic glutathione redox cycle in the liver was improved (P<0·05) in the IC group than in the IUGR group. These results were associated with lower (P<0·05) phosphorylated levels of the NF-κB pathway and Janus kinase 2 (JAK2) and higher (P<0·05) mRNA expression of genes involved in the nuclear factor, erythroid 2-like 2 (Nfe2l2)/antioxidant response element (ARE) pathway in the liver of the IC rats than that of the IUGR rats. Maternal undernutrition decreased birth weight and led to inflammation, oxidative damage and injury in rats. Curcumin appeared to be beneficial in preventing IUGR-induced inflammation, oxidative damage and injury by activating the expression of the NF-κB, JAK/STAT and Nfe2l2/ARE pathways in the liver.