Cytological Studies of Early Stages of Powdery Mildew in Barley and Wheat Leaves
Cytological Studies of Early Stages of Powdery Mildew in Barley and Wheat Leaves
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大麦和小麦叶片白粉病早期的细胞学研究
DOI：
10.3186/jjphytopath.43.202
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
H. Kunoh
中科院分区：
文献类型：
--
作者：
T. Tsuzuki;H. Ishizaki;H. Kunoh
在许多植物中，乳头的形成是一种常见的细胞对真菌攻击的反应。据报道，这种反应是由机械和化学刺激1、2、3引起的。最近，我们发现吐温20和/或甘油也能诱导大麦叶片表皮形成伤口插件和晕圈。用组织化学显色反应和扫描电子显微镜观察了这些化学物质引起的伤口塞子和晕圈，并与白粉病侵染引起的乳头和晕圈进行了比较。大麦(Hordeum visgare L.cv.)Kobinkatagi)是在最近的荧光灯(约4000勒克斯)照亮的室内每天12小时内从种子中培育出来的。小室保持在20℃和70%相对湿度。10天后，用剃须刀片从根部切下初生叶，并将其正面朝下放入培养皿中。树叶的两端被湿润的棉花覆盖。将白粉菌的分生孢子接种到部分叶片的叶面上。霍尔代人，第一小种(与cv.Kobinkatagi)，而其他叶片的叶面喷施0.05%(V/V)吐温20、2%甘油或0.05%吐温20和2%甘油的混合物，距离约10 cm。培养皿盖上盖子以保持较高的相对湿度，并放置在20℃和90%相对湿度的受控环境室中。接种24小时后，一些接种或喷洒的叶片的表皮被剥离，并用下面引用的染料染色。4)用戊二醛、单宁酸和四氧化三铯进行电导染色，用日立HHS-2X扫描电子显微镜不涂膜观察。用装在SEM5中的机械手解剖标本)。
Papilla formation in many plant species is a common cell response to fungal attacks. Such a response has reportedly been incited by mechanical and chemical stimuli1, 2, 3). Recently, we found that Tween 20 and/or glycerine also induced wound plugs3) and halo formation in barley leaf epidermis. In this paper, we carried out histochemical color reactions and scanning electron microscopic observations of wound plugs and halos induced by these chemicals in comparison to papillae and halos induced by powdery mildew infection. Barley (Hordeum vulgare L. cv. Kobinkatagi) was grown from seed in a chamber lit by fluorecent lamps (ca. 4,000 lux) for 12hrs per day. The chamber was maintained at 20C and 70%RH. After 10 days, primary leaves were cut off at the bases with a razor blade and placed adaxial surface down in petri dishes. Cut ends of the leaves were covered with moistened cotton. Abaxial surfaces of some leaves were inoculated with conidia of Erysiphe graminis f. sp. hordei, race I (compatible with cv. Kobinkatagi), and those of other leaves were sprayed with 0.05% (V/V) Tween 20, 2% glycerine, or a mixture of 0.05% Tween 20 and 2% glycerine, from a distance of about 10cm. The petri dishes were covered with a lid to maintain high RH and placed in a controlled environment chamber at 20C and 90% RH. Twenty-four hrs after inoculation, epidermises of some of the inoculated or sprayed leaves were stripped and stained with dyes cited below. The other leaves were fixed by the conductive staining method4) using glutaraldehyde, tannic acid, and osmium tetraoxide, then observed with a Hitachi scanning electron microscope HHS-2X without coating. The specimens were dissected with a manipulator set in the SEM5).