NUCLEOTIDE-SEQUENCE OF THE ORIGIN OF REPLICATION OF THE ESCHERICHIA-COLI K-12 CHROMOSOME
NUCLEOTIDE-SEQUENCE OF THE ORIGIN OF REPLICATION OF THE ESCHERICHIA-COLI K-12 CHROMOSOME
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DOI：
10.1073/pnas.76.2.580
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
SCHALLER, H
中科院分区：
文献类型：
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作者：
MEIJER, M;BECK, E;SCHALLER, H
大肠杆菌染色体的复制起点oriC被定位在一个422个碱基对的DNA片段内。这个片段的核苷酸序列已被确定。用于序列分析的DNA来源是一种在体内构建的微型染色体，它完全由染色体DNA组成，以及一种通过体外克隆构建的微型染色体。复制起点的核苷酸序列由于反向重复和正向重复而具有高度的重复性特征。在大肠杆菌与λ噬菌体和G4噬菌体的复制起点部分之间发现了序列同源性。这表明不同复制子在复制起始的某些步骤上存在相似性。
The origin of replication, oriC, of the E. coli chromosome was mapped within a DNA segment of 422 base-pairs. The nucleotide sequence of this segment was determined. The source of DNA for the sequence analysis was a minichromosome constructed in vivo, consisting exclusively of chromosomal DNA and a minichromosome constructed by cloning in vitro. The nucleotide sequence of the replication origin is characterized by a high degree of repetitiveness due to both inverted and direct repeats. Sequence homologies were found between portions of the replication origins of E. coli and phages .lambda. and G4. This suggests similarities in some steps in the initiation of replication of the different replicons.