PROPERTIES OF A MONOCLONAL-ANTIBODY TO EPIDERMAL GROWTH-FACTOR RECEPTOR WITH IMPLICATIONS FOR THE MECHANISM OF ACTION OF EGF
PROPERTIES OF A MONOCLONAL-ANTIBODY TO EPIDERMAL GROWTH-FACTOR RECEPTOR WITH IMPLICATIONS FOR THE MECHANISM OF ACTION OF EGF
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DOI：
10.1002/j.1460-2075.1984.tb01910.x
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发表时间：
1984-01-01
期刊：
影响因子：
11.4
通讯作者：
REES, AR
中科院分区：
文献类型：
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作者：
GREGORIOU, M;REES, AR
针对 A431 细胞（人表皮样癌）中存在的表皮生长因子 (EGF) 受体而产生的单克隆抗体 EGR/G49 通过降低主要低亲和力受体群体的亲和力来抑制 EGF 结合，同时使次要的高亲和力群体相对不受干扰。该抗体在不同于 EGF 结合位点的位点结合碳水化合物决定簇，诱导受体的聚集和内化，而不刺激 EGF 受体激酶或影响其接受 EGF 刺激的能力。它对 A431 细胞具有毒性，并诱导与这些细胞受到 1-10 nM 浓度范围的 EGF 攻击时所观察到的形态变化类似的形态变化。显然，高亲和力和低亲和力EGF受体是可以区分的，并且它们可能具有不同的功能。
A monoclonal antibody, EGR/G49, raised against the receptor for epidermal growth factor (EGF) present in A431 cells [human epidermoid carcinoma] inhibits EGF binding by decreasing the affinity of the major population of low affinity receptors while leaving the minor high affinity population relatively unperturbed. The antibody, which binds to a carbohydrate determinant at a site distinct from the EGF binding site, induces clustering and internalization of the receptor without stimulating the EGF receptor-kinase or affecting its ability to undergo stimulation by EGF. It is toxic to A431 cells and induces morphological changes similar to those seen when these cells are challenged with EGF in the concentration range 1-10 nM. Apparently, high and low affinity EGF receptors can be distinguished and they may serve different functions.