Characterization and antioxidant activity of the oligo-maltose fraction from Polygonum Cillinerve
Characterization and antioxidant activity of the oligo-maltose fraction from Polygonum Cillinerve
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DOI：
10.1016/j.carbpol.2019.115307
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发表时间：
2019-12-15
影响因子：
11.2
通讯作者：
Fan, Yunpeng
中科院分区：
文献类型：
--
作者：
Zhou, Yu;Ma, Wuren;Fan, Yunpeng
本研究采用高效凝胶渗透色谱、高效液相色谱、傅立叶变换红外光谱、核磁共振和质谱学等方法对虎杖多糖进行了研究。结果表明，PCP由葡萄糖组成，为α-D-葡聚糖，其主链由(1-和4)-α-D-葡萄糖重复单元组成。此外，还对五氯苯酚的体外抗氧化能力进行了评价。结果表明，五氯酚具有较强的清除羟基自由基的能力，对DPPH有一定的清除能力，具有较好的还原能力。此外，酶联免疫吸附试验结果显示，15.625~0.975 mU g/m L的五氯苯酚可显著提高巨噬细胞超氧化物歧化酶和谷胱甘肽过氧化物酶的水平，显著降低髓过氧化物酶、丙二醛和黄嘌呤氧化酶的水平。这些结果表明，五氯苯酚有可能被开发为一种天然抗氧化剂。
In this study, Polygonum Cillinerve polysaccharide (PCP) was extracted, and characterized by high performance gel permeation chromatography, high performance liquid chromatography, fourier transform infrared spectroscopy, nuclear magnetic resonance and mass spectroscopy. The results showed that PCP was composed of glucose, it was alpha-D-glucan and the backbone of PCP was consisted of repeating units of (1 -> 4)-alpha-D-Glucose. In addition, the antioxidant potential of PCP was assessed in vitro. The results showed that PCP had strong hydroxyl radical scavenging ability, some DPPH scavenging ability and good reducing power. Moreover, the results of the enzyme-linked immunosorbent assays showed that PCP at 15.625-0.975 mu g/mL could significantly improve the level of superoxide dismutase and glutathione peroxidase, and could significantly decrease the level of myeloperoxidase, malondialdehyde and xanthine oxidase in macrophages. These results indicated that PCP could potentially be developed as a natural antioxidant.