Using transgenic Caenorhabditis elegans in soil toxicity testing
Using transgenic Caenorhabditis elegans in soil toxicity testing
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DOI：
10.1007/s00244-004-0031-2
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发表时间：
2005-05-01
影响因子：
4
通讯作者：
Williams, PL
中科院分区：
文献类型：
--
作者：
Graves, AL;Boyd, WA;Williams, PL
土壤生物测定是评价陆地环境中毒理学效应的重要工具。美国材料与试验学会E2172-01标准指南概述了使用秀丽隐杆线虫进行实验室土壤毒性试验的方法。这种方法也是从土壤样品中提取线虫的有效方法，并且可以在24小时暴露期后使用相对少量的土壤进行。这种方法的缺点包括以下问题：（1）从含有高百分比有机物质的土壤中回收线虫，以及（2）区分本地线虫物种和为实验室测试添加的线虫。由于这些问题，C。秀丽隐杆线虫尚未被广泛接受用于土壤测试。为了解决这些问题并改进美国测试与材料协会的方法，该项目的重点是使用转基因C.携带GFP表达元件的线虫。致死率和行为测试表明，转基因线虫的响应类似于野生型N2菌株，表明它们可以以相同的方式用于土壤测试。GFP标记不仅在含有大量有机物质的土壤中容易识别，而且在含有土著线虫的田间采集的土壤中也容易识别。这些结果支持使用转基因GFP C。土壤生物测定中的秀丽隐杆线虫作为进一步提高实验室毒性测试可靠性的工具。
Soil bioassays are important tools for evaluating toxicological effects within the terrestrial environment. The American Society for Testing and Materials E2172-01 Standard Guide outlines a method for conducting laboratory soil toxicity tests using the nematode Caenorhabditis elegans. This method is an efficient too] for extracting C elegans from soil samples and can be carried out after a 24-h exposure period using relatively small amounts of soil. Drawbacks of this method include problems with (1) recovery of nematodes from soils containing a high percentage of organic matter, and (2) distinguishing indigenous nematode species from nematodes added for the laboratory test. Due in part to these issues, C. elegans has not been extensively accepted for use in soil testing. To address these concerns and improve upon the American Society for Testing and Materials method, this project focused on using transgenic strains of C. elegans carrying a GFP-expressing element. Lethality and behavior tests revealed that the transgenic nematodes respond similarly to the wild-type N2 strain, indicating that they can be used in the same manner in soil testing. The GFP marker is easily identifiable not only within soils containing a large amount of organic matter, but also in field-collected soils containing indigenous nematodes. These results support the use of transgenic GFP C. elegans in soil bioassays as a tool to further the reliability of laboratory toxicity tests.