Myosin-Va is required for preciliary vesicle transportation to the mother centriole during ciliogenesis
Myosin-Va is required for preciliary vesicle transportation to the mother centriole during ciliogenesis
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DOI：
10.1038/s41556-017-0018-7
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发表时间：
2018-02-01
影响因子：
21.3
通讯作者：
Tang, Tang K.
中科院分区：
文献类型：
--
作者：
Wu, Chien-Ting;Chen, Hsin-Yi;Tang, Tang K.
初级纤毛在信号转导和发育中起重要作用。纤毛前囊泡与中心粒末端附属物的对接是纤毛发生的起始/关键步骤，但其机制尚不清楚。在这里，我们证明，肌球蛋白Va介导的运输纤毛囊泡的母亲中心粒，并揭示了潜在的机制。我们还表明，肌球蛋白Va介导的纤毛前囊泡的运输是最早的事件，定义的纤毛发生的发病。肌球蛋白-Va的耗尽显着抑制纤毛囊泡的附着到母亲中心粒的远端附属物，并减少纤毛组装。肌球蛋白Va在EHD 1和Rab 11介导的睫状囊泡形成的上游起作用。重要的是，动力蛋白介导肌球蛋白Va相关的前纤毛囊泡运输到周围的中心体区域沿着微管，而肌球蛋白Va介导的前纤毛囊泡运输从周围的中心体区域的远端附属物的母亲中心粒通过Arp 2/3相关的分支肌动蛋白网络。
Primary cilia play essential roles in signal transduction and development. The docking of preciliary vesicles at the distal appendages of a mother centriole is an initial/critical step of ciliogenesis, but the mechanisms are unclear. Here, we demonstrate that myosin-Va mediates the transportation of preciliary vesicles to the mother centriole and reveal the underlying mechanism. We also show that the myosin-Va-mediated transportation of preciliary vesicles is the earliest event that defines the onset of ciliogenesis. Depletion of myosin-Va significantly inhibits the attachment of preciliary vesicles to the distal appendages of the mother centriole and decreases cilia assembly. Myosin-Va functions upstream of EHD1- and Rab11-mediated ciliary vesicle formation. Importantly, dynein mediates myosin-Va-associated preciliary vesicle transportation to the pericentrosomal region along microtubules, while myosin-Va mediates preciliary vesicle transportation from the pericentrosomal region to the distal appendages of the mother centriole via the Arp2/3-associated branched actin network.