FT-Raman spectra of the border of infiltrating ductal carcinoma lesions
FT-Raman spectra of the border of infiltrating ductal carcinoma lesions
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DOI：
10.1089/pho.2007.2094
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发表时间：
2007-10-01
影响因子：
--
通讯作者：
Martin, Airton Abrahao
中科院分区：
文献类型：
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作者：
de Miranda Marzullo, Ana Carolina;Neto, Osmar Pinto;Martin, Airton Abrahao
目的：病灶边界的FT-Raman光谱特征对指导外科医生进行手术干预具有重要意义。背景资料：本研究的主要目的是研究浸润性导管癌（IDC）病变边界的光谱，并确定这些光谱的特征。方法：利用ft -拉曼光谱采集5例正常乳腺组织和13例乳腺癌组织的93个光谱。采用聚类分析将光谱分成不同的组。组织病理学分析证实了统计分析的结果。结果：从IDC样品中收集的67个光谱中有17个表现出广泛的变化。正常组织的光谱峰与病变边界的光谱峰之间的唯一显著差异是538 cm处有一个峰。(1)该峰与半胱氨酸中的二硫桥有关，它似乎是FT-Raman测定健康组织和病理组织边界的主要因素。结论：本研究结果为拉曼光谱在光学诊断中指导活检和手术干预的进一步研究和应用奠定了基础。
Objective: The characterization of the FT-Raman spectra of the borders of lesions is of great importance in guiding the surgeon during surgical intervention. Background Data: The main goals of this study were to investigate spectra of the borders of lesions of samples of infiltrating ductal carcinoma (IDC) and to determine the characteristics of these spectra. Methods: A total of 93 spectra were collected from five samples of healthy tissues and from 13 samples of IDC breast tissues using FT-Raman spectroscopy. Cluster analysis was used to separate the spectra into different groups. The results obtained from the statistical analysis were confirmed by a histopathological analysis. Results: The results showed that 17 out of the 67 spectra collected from the IDC samples demonstrated wide variety. The only significant difference between the peaks of the spectra of normal tissues and those of lesion borders is a peak at 538 cm.(-1) This peak is related to disulfide bridges in cysteine, and it seems to be the main factor for the FT-Raman determination of the boundaries between healthy and pathological tissue. Conclusions: These results serve as a foundation for future studies and application of Raman spectroscopy for optical diagnosis to guide biopsy and surgical intervention.