Action of disinfectant quaternary ammonium compounds against Staphylococcus aureus
Action of disinfectant quaternary ammonium compounds against Staphylococcus aureus
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DOI：
10.1128/aac.00375-06
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发表时间：
2007-01-01
影响因子：
4.9
通讯作者：
Denyer, Stephen P.
中科院分区：
文献类型：
--
作者：
Ioannou, Christopher J.;Hanlon, Geoff W.;Denyer, Stephen P.
作用方式研究得出结论，烷基二甲基苄基氯化铵（ADBAC）（C1，，在35 ℃下，在1 × 10（5）至1 × 10（9）CFU/ml的接种范围内，ADBAC和DDAC在0.4 ppm至1.8 ppm范围内表现出相似的MIC行为。ADBAC和DDAC对S.与25 ℃相比，在35 ℃下观察到金黄色葡萄球菌（终浓度，2 × 10（9）CFU/ml）。两种药剂的杀伤浓度指数（eta）均< 2.5，温度影响eta值。泄漏和杀灭数据的检查表明，单个泄漏标记物并不指示细胞死亡。ADBAC和DDAC分别具有Langmuir（L4）和高亲和力（H3/4）吸收等温线。ADBAC分子在初级摄取结束时形成单层细胞覆盖，DDAC形成双层细胞覆盖。在杀菌浓度下发生快速细胞渗漏，细胞内钾和260 nm吸收池以严格的顺序释放。在9 μ g/ml（分别为0.0278 mM和0.0276 mM）及以上浓度下观察到ADBAC和DDAC的自溶，以及约30%的内部钾库的耗竭。自溶导致ADBAC和DDAC致死，尽管高杀生物剂浓度可能抑制自溶酶活性。
Mode-of-action studies concluded that alkyldimethylbenzylammonium chloride (ADBAC) (a blend of C,,, C,, and C,, alkyl homologues) and didecyldimethylammonium chloride (DDAC) are both membrane-active agents, possessing subtly different modes of action reflecting early cell interactions against Staphylococcus aureus. ADBAC and DDAC exhibited similar MIC behaviors from 0.4 ppm to 1.8 ppm over an inoculum range of 1 x 10(5) to 1 x 10(9) CFU/ml at 35 degrees C. For ADBAC and DDAC, an increased rapidity of killing against S. aureus (final concentration, 2 X 10(9) CFU/ml) was observed at 35 degrees C compared to 25 degrees C. Concentration exponents (eta) for killing were < 2.5 for both agents, and temperature influenced the eta value. Examination of leakage and kill data suggested that a single leakage marker was not indicative of cell death. ADBAC and DDAC possessed Langmuir (L4) and high-affinity (H3/4) uptake isotherms, respectively. ADBAC molecules formed a single monolayer of coverage of cells at the end of primary uptake, and DDAC formed a double monolayer. Rapid cell leakage occurred at bactericidal concentrations, with total depletion of the intracellular potassium and 260-nm-absorbing pools released in this strict order. Autolysis was observed for ADBAC and DDAC at concentrations of 9 mu g/ml (0.0278 mM and 0.0276 mM, respectively) and above, together with the depletion of approximately 30% of the internal potassium pool. Autolysis contributed to ADBAC and DDAC lethality, although high biocide concentrations may have inhibited autolytic enzyme activity.