Plasma Cholesterol-Lowering Activity of Gingerol- and Shogaol-Enriched Extract Is Mediated by Increasing Sterol Excretion
Plasma Cholesterol-Lowering Activity of Gingerol- and Shogaol-Enriched Extract Is Mediated by Increasing Sterol Excretion
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DOI：
10.1021/jf5043344
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发表时间：
2014-10-29
影响因子：
6.1
通讯作者：
Chen, Zhen-Yu
中科院分区：
文献类型：
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作者：
Lei, Lin;Liu, Yuwei;Chen, Zhen-Yu
本研究调查了姜酚和姜烯酚丰富的生姜提取物（GSE）的降胆固醇活性。将30只仓鼠分为三组，分别饲喂对照饲料或含0.5%和1.0% GSE的两种实验饲料之一。血浆总胆固醇、肝脏胆固醇和主动脉粥样硬化斑块随着饲料中添加GSE量的增加而呈剂量依赖性降低。粪固醇分析表明，膳食GSE以剂量依赖性方式增加中性和酸性固醇的排泄。GSE下调肠道尼曼-匹克C1样1蛋白（NPC 1 L1）、酰基CoA：胆固醇酰基转移酶2（ACAT 2）、微粒体三酰甘油转运蛋白（MTP）和ATP结合盒转运蛋白5（ABCG 5）的mRNA水平，而上调肝脏胆固醇-7 α-羟化酶（CYP 7A 1）。得出的结论是，膳食GSE对脂蛋白谱的有益改变是通过上调肝脏CYP 7A 1和下调肠道NPC 1 L 1、ACAT 2和MTP的mRNA来增强粪便胆固醇和胆汁酸的排泄来介导的。
The present study investigated the cholesterol-lowering activity of gingerol- and shogaol-enriched ginger extract (GSE). Thirty hamsters were divided into three groups and fed the control diet or one of the two experimental diets containing 0.5 and 1.0% GSE. Plasma total cholesterol, liver cholesterol, and aorta atherosclerotic plaque were dose-dependently decreased with increasing amounts of GSE added into diets. The fecal sterol analysis showed dietary GSE increased the excretion of both neutral and acidic sterols in a dose-dependent manner. GSE down-regulated the mRNA levels of intestinal Niemann-Pick C1-like 1 protein (NPC1L1), acyl CoA:cholesterol acyltransferase 2 (ACAT2), microsomal triacylglycerol transport protein (MTP), and ATP binding cassette transporter 5 (ABCG5), whereas it up-regulated hepatic cholesterol-7 alpha-hydroxylase (CYP7A1). It was concluded that beneficial modification of the lipoprotein profile by dietary GSE was mediated by enhancing excretion of fecal cholesterol and bile acids via up-regulation of hepatic CYP7A1 and down-regulation of mRNA of intestinal NPC1L1, ACAT2, and MTP.