Preincubation with green tea polyphenol extract is beneficial for attenuating sperm injury caused by freezing-thawing in swine.
Preincubation with green tea polyphenol extract is beneficial for attenuating sperm injury caused by freezing-thawing in swine.
复制标题
绿茶多酚提取物预孵育有利于减轻冻融对猪精子的损伤。
DOI：
10.1111/asj.12379
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发表时间：
2015
影响因子：
2
通讯作者：
Miyoshi K
中科院分区：
文献类型：
--
作者：
Kitaji H;Ookutsu S;Sato M;Miyoshi K
据报道，从绿茶中提取的多酚（PFs）是一种有效的抗氧化剂，可以有效地提高哺乳动物精子的活力和活力，并以液体形式保存。因此，我们测试了PFs是否也能有效地保持冻融野猪精子的完整性。将收集的小爪猪的射精液在含有不同数量的PFs（0、0.01、0.05、0.1和0.2% w/v）的精液填充剂中稀释，然后在15°C下保存过夜。使用秸秆冷冻程序对精液样本进行处理，然后在液氮中冷冻。在40°C快速解冻后，精子进行几项检测以评估精液质量。在含有0.01% w/v PFs的培养基中冷冻的精子解冻后的活力和顶体完整性显著高于不含PFs的精子（P< 0.05）。然而，两组之间的线粒体活性没有变化。添加0.01% PFs对提高单精子卵母细胞形成率和囊胚形成率均有显著（P< 0.05）的作用。这些研究结果表明，在冷冻前使用含有0.01% PFs的精液填充剂进行预孵育，可以通过防止冻融相关的损伤来保护野猪精子。
Polyphenols (PFs) extracted from green tea, known to be potent anti‐oxidants, have been reported to be effective in increasing the motility and viability of mammalian sperm, preserved in a liquid form. Therefore, we tested whether PFs might also be effective for maintaining the integrity of frozen‐thawed boar spermatozoa. Ejaculates, collected from Clawn miniature pigs, were diluted in a semen extender containing various amounts of PFs (0, 0.01, 0.05, 0.1 and 0.2% w/v) and then stored at 15°C overnight. The semen samples were processed, using the straw freezing procedure, and then frozen in liquid nitrogen. After rapid thawing at 40°C, the spermatozoa were subjected to several assays to evaluate semen quality. Spermatozoa frozen in a medium containing 0.01% w/v PFs exhibited significantly (P< 0.05) higher degrees of post‐thawed viability and acrosomal integrity than those stored in the absence of PFs. However, no change in the mitochondrial activity was noted between the two groups. The inclusion of 0.01% PFs in the semen extender was significantly (P< 0.05) effective in increasing both the rates of monospermic oocyte formation and of blastocyst formation. These findings indicate that preincubation with the semen extender, containing 0.01% PFs prior to freezing, exerts a protective effect on boar sperm by preventing injuries associated with freezing‐thawing.