Vibrio sagamiensis sp. nov., luminous marine bacteria isolated from sea water.
Vibrio sagamiensis sp. nov., luminous marine bacteria isolated from sea water.
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DOI：
10.2323/jgam.56.499
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
Susumu Yoshizawa;M. Wada;A. Yokota;K. Kogure
中科院分区：
文献类型：
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作者：
Susumu Yoshizawa;M. Wada;A. Yokota;K. Kogure
分别从日本相模湾和赤道地区的海水中分离出两种新型海洋发光细菌LC2-047T和LC1-908。这些细胞是革兰氏阴性、杆状且能运动，具有一根极鞭毛。这些细菌呈氧化酶阳性和过氧化氢酶阳性，能够在 0.5-6% NaCl（最佳 3-5%）和 10-37°C（最佳 25-30°C）温度下生长。基于16S rRNA基因序列的系统发育分析表明，这些细菌与天青弧菌（序列相似性为99.4％）和副溶血弧菌（序列相似性为99.3％）密切相关。 DNA-DNA 杂交表明 LC2-047T 和 LC1-908 是同种的，并且与最近的亲属明显分开，DNA-DNA 杂交相似性低于 60%。使用六个基因座（gyrB、pyrH、gapA、ftsZ、mreB 和 recA）的多位点序列分析 (MLSA) 显示这些细菌属于 Harvey 进化枝。此外，这些菌株与最相关的弧菌物种的不同之处在于利用葡萄糖、甘露醇、肌醇、蔗糖和苦杏仁苷以及产生酯酶(C4)、脂肪酶(C4)和酸性磷酸酶。主要脂肪酸是C15:0异2-OH和/或C16:1ω7c、C16:0、C18:1ω7c、C14:0、C15:0、C17:1ω8c和C12:0。 LC2-047T和LC1-908的DNA G+C含量分别为43.8和43.7mol%。根据本研究中提出的多相分类学证据，得出结论，菌株 LC2-047T 和 LC1-908 属于相同的基因种，代表弧菌属的一个新种，其名称为 Vibrio sagamiensis sp。十一月被提议。典型菌株为 LC2-047T (=NBRC 104589T = KCTC 22354T)。弧菌科包括六个属：弧菌属（Baumann 和 Schubert，1984）、发光杆菌属（Baumann 和 Baumann，1984）、盐弧菌属（Mellado 等人，1996）、Grimonita（Thompson 等人，2003a）、肠弧菌属（Thompson 等人，2002）和 Aliivibrio （Urbanczyk 等人，2007）。弧菌属的几种物种在水生环境中普遍存在，特别是在海洋中，并且通常与从浮游生物到鱼类等各种生物体分离（Thompson 等，2003b）。最近基于多位点序列分析 (MLSA) 的分子生物学技术显示，弧菌属包含 14 个不同的进化枝：Anguillarum、Cholerae、Corallilyticus、Diazotropicicus、Gazogenes、Fischeri、Halioticoli、Harveyi、Nereis、Nigripulchritudo、Orientalis、Scopthalmi、Splendidus 和 Vulnifi cus（Sawabe 等人， 2007）。现在 Fischeri 分支被认为是 J. Gen. Appl 属。微生物学, 56, 499-507 (2010)
Two novel marine luminous bacteria, LC2-047T and LC1-908, were isolated from seawater in Sagami Bay in Japan and an equatorial area, respectively. The cells are Gram-negative, rod shaped and motile with one polar flagellum. These bacteria are oxidase-positive and catalase-positive, and are able to grow in 0.5‒6% NaCl (optimum 3‒5%) and at temperatures of 10‒37°C (optimum 25‒30°C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that these bacteria were closely related to Vibrio azureus (99.4% sequence similarity) and Vibrio parahaemolyticus (99.3%). DNA‒DNA hybridization demonstrated that LC2-047T and LC1-908 were conspecific and clearly separated from the closest relative with less than 60% DNA‒DNA hybridization similarity. Multilocus sequence analysis (MLSA) using six loci (gyrB, pyrH, gapA, ftsZ, mreB, and recA) showed that these bacteria are included in the Harvey clade. Additionally, these strains differ from the closest related Vibrio species by the utilization of glucose, mannitol, inositol, sucrose and amygdalin and production of esterase (C4), lipase (C4) and acid phosphatase. The major fatty acids are C15:0 iso 2-OH and/or C16:1ω7c, C16:0, C18:1ω7c, C14:0, C15:0, C17:1ω8c and C12:0. The DNA G+C content of LC2-047T and LC1-908 were 43.8 and 43.7 mol%, respectively. On the basis of the polyphasic taxonomic evidence presented in this study, it is concluded that strains LC2-047T and LC1-908 belong to the same genospecies and represent a novel species of the genus Vibrio, for which the name Vibrio sagamiensis sp. nov. is proposed. The type strain is LC2-047T (=NBRC 104589T = KCTC 22354T). The family Vibrionaceae includes six genera: Vibrio (Baumann and Schubert, 1984), Photobacterium (Baumann and Baumann, 1984), Salinivibrio (Mellado et al., 1996), Grimonita (Thompson et al., 2003a), Enterovibrio (Thompson et al., 2002) and Aliivibrio (Urbanczyk et al., 2007). Several species of the genus Vibrio are ubiquitous in aquatic environments, especially in the ocean, and are often isolated from various organisms ranging from plankton to fish (Thompson et al., 2003b). A recent molecular biological technique based on Multilocus sequence analysis (MLSA) showed that genus Vibrio comprises 14 distinct clades: Anguillarum, Cholerae, Coralliilyticus, Diazotrophicus, Gazogenes, Fischeri, Halioticoli, Harveyi, Nereis, Nigripulchritudo, Orientalis, Scopthalmi, Splendidus and Vulnifi cus (Sawabe et al., 2007). Now Fischeri clade is regarded as genus J. Gen. Appl. Microbiol., 56, 499‒507 (2010)