Modulation of KGF-1 gene expression in oral fibroblasts by ripe areca nut extract
Modulation of KGF-1 gene expression in oral fibroblasts by ripe areca nut extract
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DOI：
10.1034/j.1600-0714.2003.00133.x
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发表时间：
2003-08-01
影响因子：
3.3
通讯作者：
Liu, TY
中科院分区：
文献类型：
--
作者：
Ko, SY;Lin, SC;Liu, TY
背景：咀嚼槟榔是亚洲人的一种常见习惯，导致该人群中各种口腔疾病的高倾向性。本研究旨在研究成熟槟榔提取物（rANE）对口腔成纤维细胞系基因表达水平的影响。方法：建立15株20 ~ 77岁成人口腔成纤维细胞系。用40马克杯/毫升rANE处理细胞系24小时，获得用于cDNA芯片分析的RNA。结果：在检测到表达水平的320个基因中，14个基因上调，26个基因下调，上调幅度超过2.5倍。半定量RT-PCR分析显示，多种细胞系IL-6表达上调，PDGFR、APP-1和KGF-1表达下调，与微阵列分析结果一致。通过实时定量RT-PCR分析，发现60%（9/15）的细胞系在40杯/毫升rANE的作用下，KGF-1表达显著下调，幅度为对照组的1.5- 9倍。结论：本研究建立了一个新的rANE毒理基因组数据库。口腔成纤维细胞系中KGF-1表达的下调可能会损害其上覆盖的角化细胞的增殖，这可以部分解释慢性槟榔咀嚼者体内经常观察到的上皮萎缩。
Background: Areca chewing is a common habit of Asians, leading to a high propensity for a variety of oral diseases in this population. This research aimed to study the expression level of genes in oral fibroblast cell lines in response to exposure to ripe areca nut extract (rANE).Methods: Fifteen oral fibroblast cell lines obtained from individuals aged 20-77 years were established. Treatment of a cell line with 40 mug/ml rANE for 24 h was performed to achieve RNA for cDNA microarray analysis.Results: Among some 320 genes exhibiting detectable expression levels, 14 were up-regulated and 26 were down-regulated more than 2.5-fold. Semi-quantitative RT-PCR analysis suggested that up-regulation of IL-6 expression and down-regulation of PDGFR , APP-1 and KGF-1 expressions in multiple cell lines assayed, were compatible with the results of the microarray analysis. Using quantitative real-time RT-PCR analysis, a remarkable down-regulation of KGF-1 expression in response to 40 mug/ml rANE, ranging 1.5-ninefold as compared to controls, was found in 60% (9/15) of the cell lines.Conclusion: This study established a novel toxicogenomic database for rANE. The down-regulation of KGF-1 expression in oral fibroblast cell lines potentially impairs the proliferation of overlying keratinocytes, which could partially explain the frequent epithelial atrophy observed in chronic areca chewers in vivo.