Optimization of Fermentation Conditions and Rheological Properties of Exopolysaccharide Produced by Deep-Sea Bacterium Zunongwangia profunda SM-A87
Optimization of Fermentation Conditions and Rheological Properties of Exopolysaccharide Produced by Deep-Sea Bacterium Zunongwangia profunda SM-A87
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深海细菌Zunongwangia profunda SM-A87胞外多糖发酵条件及流变特性优化
DOI：
10.1371/journal.pone.0026825
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发表时间：
2011-11-11
期刊：
影响因子：
3.7
通讯作者：
Zhang, Yu-Zhong
中科院分区：
文献类型：
--
作者：
Liu, Sheng-Bo;Qiao, Li-Ping;Zhang, Yu-Zhong
从深海沉积物中分离得到的Zunongwangia profunda SM-A87能够分泌大量的胞外多糖(EPS)。应用响应面法优化 EPS 生产的培养条件。单因素实验表明乳糖是最好的碳源。基于Plackett-Burman设计，选择乳糖、蛋白胨和温度作为显着变量，通过最陡上升（下降）法和中心组合设计进一步优化。 EPS生产和肉汤粘度的最佳培养条件确定为32.21 g/L乳糖、8.87 g/L蛋白胨和9.8°C的培养温度。在此条件下，EPS的最大产量和肉汤粘度分别为8.90 g/L和6551 mPa•s，这是海洋细菌EPS如此高产量的首次报道。 EPS的水溶液表现出高粘度、有趣的剪切稀化特性以及对高温、宽pH范围和高盐度的良好耐受性。
Zunongwangia profunda SM-A87 isolated from deep-sea sediment can secrete large quantity of exopolysaccharide (EPS). Response surface methodology was applied to optimize the culture conditions for EPS production. Single-factor experiment showed that lactose was the best carbon source. Based on the Plackett–Burman design, lactose, peptone and temperature were selected as significant variables, which were further optimized by the steepest ascent (descent) method and central composite design. The optimal culture conditions for EPS production and broth viscosity were determined as 32.21 g/L lactose, 8.87 g/L peptone and an incubation temperature of 9.8°C. Under these conditions, the maximum EPS yield and broth viscosity were 8.90 g/L and 6551 mPa•s, respectively, which is the first report of such high yield of EPS from a marine bacterium. The aqueous solution of the EPS displayed high viscosity, interesting shearing thinning property and great tolerance to high temperature, a wide range of pH, and high salinity.