Characterization of multi-cellular dynamics of angiogenesis and vascular remodelling by intravital imaging of the wounded mouse cornea.
Characterization of multi-cellular dynamics of angiogenesis and vascular remodelling by intravital imaging of the wounded mouse cornea.
复制标题
通过受伤小鼠角膜的活体成像来表征血管生成和血管重塑的多细胞动力学。
DOI：
10.1038/s41598-018-28770-7
复制
发表时间：
2018
影响因子：
4.6
通讯作者：
Jakobsson,Lars
中科院分区：
文献类型：
--
作者：
Wang,Yixin;Jin,Yi;Laviña,Bàrbara;Jakobsson,Lars
功能性血管系统的建立涉及由生长因子、趋化因子和流动介导的剪切力控制的广泛的细胞重排。用阿拉伯语记录这些高度动态的过程在技术上要求很高。在这里，我们应用共聚焦和宽视野的时间lapsein活显微镜来表征重建血管的受伤的小鼠角膜。使用组成型或诱导型内源性荧光报告基因的小鼠品系，结合示踪剂注射和嵌合遗传重组，我们在亚细胞分辨率下跟踪萌芽血管生成、萌芽融合、血管扩张和修剪的体内过程。我们描述了灌注血管内皮细胞的迁移行为，与血流方向性和血管身份。血管内注射荧光示踪剂后的实时成像，允许记录VEGF诱导的渗透性。总之，携带内源性荧光报告基因和条件等位基因的小鼠发炎角膜的重塑血管系统的实时成像构成了研究细胞行为和血管功能的强大平台。
Establishment of the functional blood vasculature involves extensive cellular rearrangement controlled by growth factors, chemokines and flow-mediated shear forces. To record these highly dynamic processes in mammalians has been technically demanding. Here we apply confocal and wide field time-lapsein vivomicroscopy to characterize the remodelling vasculature of the wounded mouse cornea. Using mouse lines with constitutive or inducible endogenous fluorescent reporters, in combination with tracer injections and mosaic genetic recombination, we follow processes of sprouting angiogenesis, sprout fusion, vessel expansion and pruningin vivo, at subcellular resolution. We describe the migratory behaviour of endothelial cells of perfused vessels, in relation to blood flow directionality and vessel identity. Live-imaging following intravascular injection of fluorescent tracers, allowed for recording of VEGFA-induced permeability. Altogether, live-imaging of the remodelling vasculature of inflamed corneas of mice carrying endogenous fluorescent reporters and conditional alleles, constitutes a powerful platform for investigation of cellular behaviour and vessel function.